Placenta and Trophoblast

Methods and Protocols Volume 1

  • Michael J. Soares
  • Joan S. Hunt

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 121)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Michael J. Soares, Joan S. Hunt
      Pages 3-6
  3. Methods for Studying Embryo Implantation and Uterine Biology

    1. Front Matter
      Pages 7-7
    2. Kaushik Deb, Jeff Reese, Bibhash C. Paria
      Pages 9-34
    3. D. Randall Armant
      Pages 35-56
    4. Yan Gu, Geula Gibori
      Pages 69-78
    5. Graciela Krikun, Gil Mor, Charles Lockwood
      Pages 79-83
    6. Thomas E. Spencer, C. Allison Gray
      Pages 85-94
    7. Asgerally T. Fazleabas
      Pages 95-99
    8. Asgerally T. Fazleabas
      Pages 101-110
    9. Almuth Einspanier, Kai Lieder, Ralf Einspanier, Bettina Husen
      Pages 111-121
  4. In Vitro Trophoblast and Placental Model Systems

    1. Front Matter
      Pages 123-123
    2. Jennifer Quinn, Tilo Kunath, Janet Rossant
      Pages 125-148
    3. Mark Kibschull, Elke Winterhager
      Pages 149-158
    4. Namita Sahgal, Lindsey N. Canham, Brent Canham, Michael J. Soares
      Pages 159-178
    5. Kazuyoshi Hashizume, Arata Shimada, Haruo Nakano, Toru Takahashi
      Pages 179-188
    6. Margaret G. Petroff, Teresa A. Phillips, Hakhyun Ka, Judith L. Pace, Joan S. Hunt
      Pages 203-217

About this book

Introduction

Placenta research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of the trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.
Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of pregnancy.

Keywords

Implantat cells development electron microscope microscope

Editors and affiliations

  • Michael J. Soares
    • 1
  • Joan S. Hunt
    • 2
  1. 1.Institute of Maternal-Fetal Biology Division of Cancer and Developmental Biology Department of Pathology and Laboratory MedicineUniversity of Kansas Medical CenterKansas City
  2. 2.Department of Anatomy and Cell BiologyUniversity of Kansas Medical CenterKansas City

Bibliographic information

  • DOI https://doi.org/10.1385/1592599834
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-404-3
  • Online ISBN 978-1-59259-983-7
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
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