Abstract
Pregnancy begins with fertilization of the ovulated oocyte by the sperm. After fertilization, the egg undergoes time-dependent mitotic division while trying to reach the blastocyst stage and the uterus for implantation. Uterine preparation for implantation is regulated by coordinated secretions and functions of ovarian sex steroids. The first sign of contact between the blastocyst and the uterus can be detected experimentally by an intravenous blue dye injection as early as the end of day 4 or the beginning of day 5 of pregnancy. This blastocyst-uterine attachment reaction leads to stromal decidual reaction only at sites of implantation. The process of implantation can be postponed and reinstated experimentally by manipulating ovarian estrogen secretion. Stromal decidualization can also be induced experimentally in the hormonally prepared uterus in response to stimuli other than the embryo. Fundamental biological questions surrounding these essential features of early pregnancy can be addressed through the application of various techniques and manipulation of this period of early pregnancy. This chapter describes the routine laboratory methodologies to study the events of early pregnancy, with special emphasis on the implantation process in mice.
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References
Allen, E. (1922) The estrous cycle in the mouse. Am. J. Anat. 30, 297ā371.
Snell, G. D., Fekete, E., Hummel, K. P., and Law, L. W. (1940) The relation of mating, ovulation and estrous smear in the house mouse to time of day. Anat. Rec. 76, 39ā54.
Carson, D. D., Bagchi, I., Dey, S. K., et al. (2000) Embryo implantation. Dev. Biol. 223, 217ā237.
Dey, S. K. (1996) Implantation. In: Reproductive Endocrinology, Surgery and Technology (Adashi, E. Y., Rock, J. A., and Rosenwaks, Z, Eds.), Lippincott-Raven, New York: pp. 421ā434.
Yoshinaga, K. and Adams, C. E. (1966) Delayed implantation in spayed, progesterone treated adult mouse. J. Reprod. Fert. 12, 593ā595.
Das, S. K., Wang, X. N., Paria, B. C., et al. (1994) Heparin-binding EGF like growth factor gene is induced in the mouse uterus temporally by blastocysts solely at the site of its apposition: a possible ligand for interaction with blastocyst EGFreceptor in implantation. Development 120, 1071ā1083.
Psychoyos, A. (1973) Endocrine control of egg implantation. In: Handbook of Physiology, Williams and Wilkins, Baltimore, MD: pp.187ā215.
Paria, B. C., Huet-Hudson, Y. M., and Dey, S. K. (1993) Blastocystās state of activity determines the āwindowā of implantation in the mouse receptive uterus. Proc. Natl. Acad. Sci. USA 90, 10,159ā10,162.
Paria, B. C., Tan, J., Lubahn, D. B., Dey, S. K., and Das, S. K. (1999) Uterine decidual response occurs in estrogen receptor-Ī±-deficient mice. Endocrinology 140, 2704ā2710.
Wordinger, R. J., Jackson, F. L., and Morrill, A. (1986) Implantation, deciduoma formation and live births in mast cell-deficient mice (W/Wv). J. Reprod. Fertil. 77, 471ā476.
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Deb, K., Reese, J., Paria, B.C. (2006). Methodologies to Study Implantation in Mice. In: Soares, M.J., Hunt, J.S. (eds) Placenta and Trophoblast. Methods in Molecular Medicineā¢, vol 121. Humana Press. https://doi.org/10.1385/1-59259-983-4:007
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DOI: https://doi.org/10.1385/1-59259-983-4:007
Publisher Name: Humana Press
Print ISBN: 978-1-58829-404-3
Online ISBN: 978-1-59259-983-7
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