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Analysis of Herbicides in Water by On-Line In-Tube Solid-Phase Microextraction Coupled With Liquid Chromatography-Mass Spectrometry

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Pesticide Protocols

Part of the book series: Methods in Biotechnology ((MIBT,volume 19))

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Abstract

We have developed a method using in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry (SPME-LC-MS) for the assay of herbicides in water samples. Chlorinated phenoxy acid herbicides have been automatically extracted into a DB-WAX capillary by repeated draw/eject cycles of sample solution and desorbed with acetonitrile. Triazine herbicides have also been extracted easily by this in-tube SPME method. Compared with direct sample injection into LC-MS, the in-tube SPME technique resulted in a greater than 25-fold concentration. The automated in-tube SPME-LC-MS system can continuously extract herbicides from aqueous samples, followed by LC-MS. It provides a simple, rapid, selective, sensitive, low-cost method for herbicide analyses and can be directly applied to various water samples without any pretreatment. Although the in-tube SPME technique has not yet been extensively applied, its application to various fields is expected because the analytical processes, from sample preparation to chromatography and data analysis, can be automated.

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Author information

Authors and Affiliations

  1. Laboratory of Applied Analytical Chemistry, Department of Biological Pharmacy, School of Pharmacy, Shujitsu University, Okayama, Japan

    Hiroyuki Kataoka & Kurie Mitani

  2. Yokogawa Analytical Systems Inc., Tokyo, Japan

    Masahiko Takino

Authors
  1. Hiroyuki Kataoka
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  2. Kurie Mitani
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  3. Masahiko Takino
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Editor information

Editors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Sciences, University of Almería, Almería, Spain

    José L. Martínez Vidal  & Antonia Garrido Frenich  & 

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© 2006 Humana Press Inc.

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Kataoka, H., Mitani, K., Takino, M. (2006). Analysis of Herbicides in Water by On-Line In-Tube Solid-Phase Microextraction Coupled With Liquid Chromatography-Mass Spectrometry. In: Martínez Vidal, J.L., Frenich, A.G. (eds) Pesticide Protocols. Methods in Biotechnology, vol 19. Humana Press. https://doi.org/10.1385/1-59259-929-X:365

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  • DOI: https://doi.org/10.1385/1-59259-929-X:365

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-410-4

  • Online ISBN: 978-1-59259-929-5

  • eBook Packages: Springer Protocols

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