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Application of Microwave-Assisted Extraction for the Analysis of Dithiocarbamates in Food Matrices

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Pesticide Protocols

Part of the book series: Methods in Biotechnology ((MIBT,volume 19))

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Abstract

Microwave-assisted extraction (MAE) is a simple, fast, and accurate method developed for the analysis of N,N-dimethyldithiocarbamate (DMDTC) and ethylenebis(dithiobamate) (EBDTC) fungicides in fruits and vegetables. Residues are extracted from the plant matrices and hydrolyzed to CS2 in a single step in the presence of 1.5% SnCl2 in 5N hydrochloric acid using a laboratory microwave oven operated in the closed-vessel mode. The evolved CS2, trapped in a layer of isooctane overlaying the reaction mixture, is analyzed by gas chromatography/flame photometric detection (GC/FPD). Sets of 12 samples are processed simultaneously. Quantification is based on external standard calibration curves made with either standard solutions of individual DMDTCs (thiram, ziram) or EBDTCs (maneb, zineb, mancozeb), which are processed as the field samples, or using standard solutions of CS2 made in isooctane. Calibration curves are better fitted with quadratic equations with correlation coefficients >0.999; however, good linear correlation coefficients can also be obtained in narrower calibration ranges. Limits of detection (LODs) and limits of quantitation (LOQs) are in the range 0.005–0.1 mg/kg. Recoveries are >80%, with respective relative standard deviation values <20%.

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References

  1. Keppel, G. E. (1969) Modification of the carbon disulfide evolution method for dithiocarbamate residues. J. AOAC 52, 162–167.

    CAS  Google Scholar 

  2. Keppel, G. E. (1971) Collaborative study of the determination of dithiocarbamate residues by a modified carbon disulfide evolution method. J. AOAC 54, 528–532.

    CAS  Google Scholar 

  3. Kesari, R. and Gupta, V. K. (1998) A sensitive spectrophotometric method for the determination of dithiocarbamate fungicide and its application in environmental samples. Talanta 45, 1097–1102.

    Article  CAS  PubMed  Google Scholar 

  4. Ahmad, N., Guo, L., Mandarakas, P., and Appleby, S. (1995) Determination of dithiocarbamate and its breakdown product ethylenethiourea in fruits and vegetables. J. AOAC Int. 78, 1238–1243.

    CAS  Google Scholar 

  5. Woodrow, J. E. and Seiber, J. N. (1995) Analytical method for the dithiocarbamate fungicides ziram and mancozeb in air: Preliminary field results. J. Agric. Food Chem. 43, 1524–1529.

    Article  CAS  Google Scholar 

  6. Ahmad, N., Guo, L., Mandarakas, P., Farah, V., Appleby, S., and Gibson, T. (1996) Headspace gas-liquid chromatographic determination of dithiocarbamate residues in fruits and vegetables with confirmation by conversion to ethylenethiourea. J. AOAC Int. 79, 1417–1422.

    CAS  PubMed  Google Scholar 

  7. Royer, A., Ménand, M., Grimault, A., and Communal, P. Y. (2001) Development of automated headspace gas chromatography determination of dithiocarbamates in plant matrixes. J. Agric. Food Chem. 49, 2152–2158.

    Article  CAS  PubMed  Google Scholar 

  8. Vryzas, Z., Papadakis, E. N., and Papadopoulou-Mourkidou, E. (2002) Microwave-Assisted Extraction (MAE)-acid hydrolysis of dithiocarbamates for trace analysis in tobacco and peaches. J. Agric. Food Chem. 50, 2220–2226.

    Article  CAS  PubMed  Google Scholar 

  9. Gustafsson, K. H. and Fahlgren, C. (1983) Determination of dithiocarbamate fungicides in vegetable foodstuffs by high-performance liquid chromatography. J. Agric. Food Chem. 31, 461–463.

    Article  CAS  PubMed  Google Scholar 

  10. Ekroth, S. B., Ohlin, B., and Österdahl, B.-G. (1998) Rapid and simple method for determination of thiram in fruits and vegetables with high-performance liquid chromatography with ultraviolet detection. J. Agric. Food Chem. 46, 5302–5304.

    Article  CAS  Google Scholar 

  11. Lo, C.-C., Ho, M.-H., and Hung, M.-D. (1996) Use of high-performance liquid chromatography and atomic absorption methods to distinguish propineb, zineb, maneb, and mancozeb fungicides. J. Agric. Food Chem. 44, 2720–2723.

    Article  CAS  Google Scholar 

  12. Weissmahr, K. W., Houghton, C. L., and Sediak, D. L. (1998) Analysis of the dithiocarbamate fungicides ziram, maneb, and zineb and the flotation agent ethylxanthogenate by ion-pair reversed-phase HPLC. Anal. Chem. 70, 4800–4804.

    Article  CAS  Google Scholar 

  13. Steinheimer, T. R. (1993) HPLC determination of atrazine and principal degradates in agricultural soils and associated surface and ground water. J. Agric. Food Chem. 41, 588–595.

    Article  CAS  Google Scholar 

  14. Molins, C., Hogendoorn, E.A., Heusinkveld, H. A. G., van Harten, D. C., van Zoonen, P., and Baumann, R.A. (1996) Microwave assisted solvent extraction for the efficient determination of triazines in soil samples with aged residues. Chromatographia 43, 527–532.

    Article  CAS  Google Scholar 

  15. Hoogerbrugge, R., Molins, C., and Baumann, R. A. (1997) Effects of parameters on microwave assisted extraction of triazines from soil: evaluation of an optimization trajectory. Anal. Chim. Acta 348, 247–253.

    Article  CAS  Google Scholar 

  16. Xiong, G., Liang, J., Zou, S., and Zhang, Z. (1998) Microwave-assisted extraction of atrazine from soil followed by rapid detection using commercial ELISA kit. Anal. Chim. Acta 371, 97–103.

    Article  CAS  Google Scholar 

  17. Papadakis, E. N. and Papadopoulou-Mourkidou, E. (2002) Determination of metribuzin and major conversion products in soils by microwave-assisted water extraction followed by liquid chromatographic analysis of extracts. J. Chromatogr. A 962, 9–20.

    Article  CAS  PubMed  Google Scholar 

  18. Vryzas, Z. and Papadopoulou-Mourkidou, E. (2002) Determination of triazine and chloroacetanilide herbicides in soils by microwave-assisted extraction (MAE) coupled to gas chromatographic analysis with either GC-NPD or GC-MS. J. Agric. Food Chem. 50, 5026–5033.

    Article  CAS  PubMed  Google Scholar 

  19. Molins, C., Hogendoorn, E. A., Dijkman, E., Heusinkveld, H. A. G., and Baumann, R. A. (2000) Determination of linuron and related compounds in soil by microwave-assisted solvent extraction and reversed-phase liquid chromatography with UV detection. J. Chtromatogr. A 869, 487–496.

    Article  CAS  Google Scholar 

  20. Hogendoorn, E. A., Huls, R., Dijkman, E., and Hoogerbrugge, R. (2001) Microwave assisted solvent extraction and coupled-column reversed-phase liquid chromatography with UV detection-Use of an analytical restricted-access-medium column for the efficient multi-residue analysis of acidic pesticides in soils. J. Chromatogr. A 938, 23–33.

    Article  CAS  Google Scholar 

  21. Patsias, J., Papadakis, E. N., and Papadopoulou-Mourkidou, E. (2002) Analysis of phenoxyalkanoic acid herbicides and their phenolic conversion products in soil by microwave assisted solvent extraction and subsequent analysis of extracts by on-line solid-phase extraction-liquid chromatography. J. Chromatogr. A 959, 153–161.

    Article  CAS  PubMed  Google Scholar 

  22. Eskilsson, C. S. and Björklund, E. (2000) Analytical-scale microwave-assisted extraction. J. Chromatogr. A 902, 227–250.

    Article  CAS  PubMed  Google Scholar 

  23. Buldini, P. L., Ricci, L., and Sharma, J. L. (2002) Recent applications of sample preparation techniques in food analysis. J. Chromatogr. A 975, 47–70.

    Article  CAS  PubMed  Google Scholar 

  24. Camel, V. (2000) Microwave-assisted solvent extraction of environmental samples. Trends Anal. Chem. 19, 229–248.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Pesticide Science Laboratory, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Euphemia Papadopoulou-Mourkidou, Emmanuil Nikolaos Papadakis & Zisis Vryzas

Authors
  1. Euphemia Papadopoulou-Mourkidou
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  2. Emmanuil Nikolaos Papadakis
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  3. Zisis Vryzas
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Editor information

Editors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Sciences, University of Almería, Almería, Spain

    José L. Martínez Vidal  & Antonia Garrido Frenich  & 

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© 2006 Humana Press Inc.

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Papadopoulou-Mourkidou, E., Papadakis, E.N., Vryzas, Z. (2006). Application of Microwave-Assisted Extraction for the Analysis of Dithiocarbamates in Food Matrices. In: Martínez Vidal, J.L., Frenich, A.G. (eds) Pesticide Protocols. Methods in Biotechnology, vol 19. Humana Press. https://doi.org/10.1385/1-59259-929-X:319

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  • DOI: https://doi.org/10.1385/1-59259-929-X:319

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-410-4

  • Online ISBN: 978-1-59259-929-5

  • eBook Packages: Springer Protocols

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