Abstract
The development of a radioimmunological method (radioimmunoassay, RIA) for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in human body fluids such as blood serum and urine is described. It comprises three major parts. The first part deals with the generation of a polyclonal anti-2,4-D antiserum in rabbits. This includes the preparation, purification, and characterization of 2,4-D-protein conjugates (immunogens), immunization of rabbits, and evaluation of antiserum quality. A guide is presented that with high probability will lead to useful antibodies. The second part outlines the synthesis and purification of the tracer (i.e., a tritiated 2,4-D with high specific activity) that is selectively labeled in the 6-position of the aromatic moiety. The third part describes carrying out the RIA, setting up the calibration curve, cross-reactivity testing, and evaluation of matrix interferences.
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Knopp, D. (2006). Analysis of 2,4-Dichlorophenoxyacetic Acid in Body Fluids of Exposed Subjects Using Radioimmunoassay. In: Martínez Vidal, J.L., Frenich, A.G. (eds) Pesticide Protocols. Methods in Biotechnology, vol 19. Humana Press. https://doi.org/10.1385/1-59259-929-X:119
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DOI: https://doi.org/10.1385/1-59259-929-X:119
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Print ISBN: 978-1-58829-410-4
Online ISBN: 978-1-59259-929-5
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