Skip to main content

Immunocapture-Polymerase Chain Reaction

  • Protocol
Immunochemical Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 295))

  • 2410 Accesses

Abstract

Antibody capture of viruses can be used as a preparatory step in nucleic acid amplification techniques. Immunocapture of virus particles can be used to streamline and/or optimize the concentration, purification and specificity requirements of polymerase chain reaction assays.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Wetzel, T., Candresse, T., Macquaire, G., Ravelonandro, M., and Dunez, J. (1992) A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. J. Virol. Methods 39, 27–37.

    Article  PubMed  CAS  Google Scholar 

  2. Nolasco, G., de Blas, C., Torres, V., and Ponz, F. (1993) A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. J. Virol. Methods 45, 201–218.

    Article  PubMed  CAS  Google Scholar 

  3. Kokko, H. I., Kivineva, M., and Karenlampi, S. O. (1996) Singlestep immunocapture RT-PCR in the detection of raspberry bushy dwarf virus. Biotechniques 20, 842–846.

    PubMed  CAS  Google Scholar 

  4. Mumford, R. A. and Seal S. E. (1997) Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses. J. Virol. Methods 69, 73–79.

    Article  PubMed  CAS  Google Scholar 

  5. Jacobi, V., Bachand, G. D., Hamelin, R. C., and Castello J. D. (1998) Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses. J. Virol. Methods 74, 167–178.

    Article  PubMed  CAS  Google Scholar 

  6. Pasquini, G., Simeone, A. M., Conte, L., and Barba, M. (1998) Detection of plum pox virus in apricot seeds. Acta Virol. 42, 260–263.

    PubMed  CAS  Google Scholar 

  7. Sefc, K. M., Leonhardt, W., and Steinkellner, H. (2000) Partial sequence identification of grapevine-leafroll-associated virus-1 and development of a highly sensitive IC-RT-PCR detection method. J. Virol. Methods 86, 101–106.

    Article  PubMed  CAS  Google Scholar 

  8. Sharman, M., Thomas, J. E., and Dietzgen, R. G. (2000) Development of a multiplex immunocapture PCR with colourimetric detection for viruses of banana. J. Virol. Methods 89, 75–88.

    Article  PubMed  CAS  Google Scholar 

  9. Helguera, P. R., Taborda, R., Docampo, D. M., and Ducasse, D. A. (2001) Immunocapture reverse transcription-polymerase chain reaction combined with nested PCR greatly increases the detection of Prunus necrotic ring spot virus in the peach. J. Virol. Methods 95, 93–100.

    Article  PubMed  CAS  Google Scholar 

  10. Brattey, C., Badge, J. L., Burns, R., Foster, G. D., George, E., Goodfellow, H. A., et al. (2002) Potato latent virus: a proposed new species in the genus Carlavirus. Plant Pathol. 51, 495–505

    Article  CAS  Google Scholar 

  11. Ptacek, J., Skopek, J., Dedic, P., and Matousek, J. (2002) Immunocapture RT-PCR probing of potato virus Y isolates. Acta Virol. 46, 63–68.

    PubMed  CAS  Google Scholar 

  12. Badge, J., Brunt, A., Carson, R., Dagless, E., Karamagioli, M., Philips, S., et al. (1996) A carlavirus-specific PCR primer and partial nucleotide sequence provides further evidence for the recognition of cowpea mild mottle mosaic virus as a whitefly-transmitted carlavirus. Eur. J. Plant Pathol. 102, 305–310.

    Article  CAS  Google Scholar 

  13. Sambrook, J. and Russell, D. (2001) Molecular Cloning: A Laboratory Manual, 3rd edition. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Mulholland, V. (2005). Immunocapture-Polymerase Chain Reaction. In: Burns, R. (eds) Immunochemical Protocols. Methods In Molecular Biology™, vol 295. Humana Press. https://doi.org/10.1385/1-59259-873-0:281

Download citation

  • DOI: https://doi.org/10.1385/1-59259-873-0:281

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-274-2

  • Online ISBN: 978-1-59259-873-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics