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Immunochemical Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Robert Burns

    1. Antibody Unit, Scottish Agricultural Science Agency, Edinburgh, Scotland

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 295)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Immunization Strategies for Antibody Production

    • Robert Burns
    Pages 1-11

  3. Synthetic Peptides as Antigens for Antibody Production

    • David C. Hancock, Nicola J. OReilly
    Pages 13-25

  4. Production of Polyclonal Antibodies in Rabbits

    • David C. Hancock, Nicola J. OReilly
    Pages 27-39

  5. Making Hybridomas

    • Robert Burns
    Pages 41-53

  6. Growing Hybridomas

    • Gary Entrican, Gareth Young
    Pages 55-70

  7. Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments

    • Michael Hust, Stefan Dübel
    Pages 71-96

  8. Purification of Antibodies and Preparation of Antibody Fragments

    • Graham D. Kemp
    Pages 97-122

  9. Conjugation of Antibodies to Alkaline Phosphatase

    • G. Brian Wisdom
    Pages 123-126

  10. Conjugation of Antibodies to Horseradish Peroxidase

    • G. Brian Wisdom
    Pages 127-130

  11. Conjugation of Antibodies to Fluorescein or Rhodamine

    • G. Brian Wisdom
    Pages 131-134

  12. Conjugation of Antibodies to Biotin

    • James D. Hirsch, Rosaria P. Haugland
    Pages 135-154

  13. Preparation of Colloidal Gold Probes

    • David Hughes
    Pages 155-172

  14. Immunogold Probes in Light Microscopy

    • David Hughes
    Pages 173-192

  15. Immunoelectron Microscope Techniques in Plant Virus Diagnosis

    • Daphne M. Wright
    Pages 193-206

  16. Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay

    • William Jordan
    Pages 207-214

  17. Competitive Enzyme-Linked Immunosorbent Assay

    • William Jordan
    Pages 215-225

  18. Immunoblotting Techniques

    • Barbara Magi, Sabrina Liberatori
    Pages 227-253

  19. Epitope Mapping

    • Glenn E. Morris
    Pages 255-267

  20. Generation and Testing of Immunoaffinity Columns

    • Weilin L. Shelver
    Pages 269-280
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Keywords

About this book

Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s. The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. Not only are potentially harmful pathogens and toxins recognized by the immune system, but the system can be persuaded to manufacture antibodies to an astonishing array of substances. In the early days of this science, all antibodies for investigative work were produced by immunizing mammals with the substance of interest, followed by regular donor bleeds that yielded antisera. Serum produced in this way yields heterogenic populations of antibody molecules recognizing different epitopes on the target protein, which may be adequate for its intended p- poses, but can also cause problems of crossreactivity. In 1975, Kohler and Milstein reported that spleen cells from immune donor animals could be immortalized, cloned from single cells, and grown in continuous culture. This original work described the method for the production of monoclonal antibodies.

Reviews

Reviews of Previous editions:
"...a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive...will be useful to both those who already have some experience in this area and...to those who wish to embark on new techniques."
-Microbiology Today

An excellent and well arranged compilation of a range of antibody techniques....An excellent laboratory manual.-Immunoassay

Editors and Affiliations

  • Antibody Unit, Scottish Agricultural Science Agency, Edinburgh, Scotland

    Robert Burns

Bibliographic Information

  • Book Title: Immunochemical Protocols

  • Editors: Robert Burns

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598730

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-274-2Published: 13 December 2004

  • Softcover ISBN: 978-1-61737-466-1Published: 10 November 2010

  • eBook ISBN: 978-1-59259-873-1Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: X, 318

  • Number of Illustrations: 22 b/w illustrations

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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