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Preparation of Colloidal Gold Probes

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Immunochemical Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 295))

Abstract

This chapter describes the production of immunogold probes for use in immunocytochemistry at the light and electron microscope levels. The protocols provide sufficient detail to enable workers to produce immunogold probes in their own laboratories. Reagents and equipment required should be readily accessible in most modern immunochemistry facilities. The protocols include descriptions of gold sol production and subsequent gold labeling of probes such as antisera. The chapter notes give suggestions for the quality control of particle production, approaches for optimizing gold probe use, and details of the latest technological advances, such ultrasmall gold particle technology.

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Author information

Authors and Affiliations

  1. Nuffield Department of Surgery, John Radcliffe Hospital, Oxford, UK

    David Hughes

Authors
  1. David Hughes
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Editor information

Editors and Affiliations

  1. Antibody Unit, Scottish Agricultural Science Agency, Edinburgh, Scotland

    Robert Burns

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© 2005 Humana Press Inc., Totowa, NJ

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Hughes, D. (2005). Preparation of Colloidal Gold Probes. In: Burns, R. (eds) Immunochemical Protocols. Methods In Molecular Biology™, vol 295. Humana Press. https://doi.org/10.1385/1-59259-873-0:155

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  • DOI: https://doi.org/10.1385/1-59259-873-0:155

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-274-2

  • Online ISBN: 978-1-59259-873-1

  • eBook Packages: Springer Protocols

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