Abstract
The development of protocols to induce a state of durable mixed allogeneic hematopoietic chimerism to confer robust donor-specific transplant tolerance has been a major focus of the transplant community for the past decade. High levels of mixed allogeneic hematopoietic chimerism across a full major histocompatibility complex (MHC) barrier can be achieved by total myeloablation and transfusion of host and donor bone-marrow cells (1) or, as shown more recently, can be achieved with nonmyeloablative preconditioning regimes in combination with donor bone-marrow transfusions (2-4) or transfusion of purified hematopoietic stem cells (HSC) (5-7). Here we illustrate useful experimental techniques for the study of hematopoietic chimerism in rodents by describing a system in which chimerism can be induced using nonmyeloablative conditioning, short-term costimulation blockade, and transplantation of purified or cultured hematopoietic stem cells.
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Emmanouilidis, N., Larsen, C.P. (2005). Induction of Chimerism and Tolerance Using Freshly Purified or Cultured Hematopoietic Stem Cells in Nonmyeloablated Mice. In: Ludewig, B., Hoffmann, M.W. (eds) Adoptive Immunotherapy: Methods and Protocols. Methods in Molecular Medicine™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-862-5:459
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DOI: https://doi.org/10.1385/1-59259-862-5:459
Publisher Name: Humana Press
Print ISBN: 978-1-58829-406-7
Online ISBN: 978-1-59259-862-5
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