Adoptive Immunotherapy

1. Front Matter

   Pages i-xvi

   PDF

2. Exploiting Dendritic Cells for Active Immunotherapy of Cancer and Chronic Infection

   • David O’Neill, Nina Bhardwaj

   Pages 1-18

3. A Mathematical Approach for Optimizing Dendritic Cell-Based Immunotherapy

   • Gennady Bocharov, Neville J. Ford, Burkhard Ludewig

   Pages 19-33

4. Delivery of Tumor Antigens to Dendritic Cells Using Biodegradable Microspheres

   • Ying Waeckerle-Men, Bruno Gander, Marcus Groettrup

   Pages 35-46

5. RNA Transfection of Dendritic Cells

   • Frank Grünebach, Martin R. Müller, Peter Brossart

   Pages 47-54

6. Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System

   • John D. M. Campbell, Christoph Piechaczek, Gregor Winkels, Edith Schwamborn, Daniela Micheli, Sonja Hennemann et al.

   Pages 55-70

7. Generation of Clinical-Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System

   • Thomas Putz, Hubert Gander, Reinhold Ramoner, Claudia Zelle-Rieser, Andrea Rahm, Walter Nussbaumer et al.

   Pages 71-81

8. Adenoviral Transduction of Dendritic Cells

   • Rienk Offringa, Kitty Kwappenberg, Martijn Rabelink, Delphine Rea, Rob Hoeben

   Pages 83-95

9. Generation of Autologous Peptide- and Protein-Pulsed Dendritic Cells for Patient-Specific Immunotherapy

   • David O’Neill, Nina Bhardwaj

   Pages 97-112

10. Phenotypical and Functional Characterization of Clinical-Grade Dendritic Cells

    • I. Jolanda, M. de Vries, Gosse J. Adema, Cornelis J. A. Punt, Carl G. Figdor

    Pages 113-125

11. Dendritic Cells in Clinical Trials for Multiple Myeloma

    • Volker L, Reichardt and Peter Brossart

    Pages 127-135

12. Identification of Tumor-Associated Autoantigens With SEREX

    • Özlem Türeci, Dirk Usener, Sandra Schneider, Ugur Sahin

    Pages 137-154

13. Application of Proteomics and Protein Analysis for Biomarker and Target Finding for Immunotherapy

    • Petra Weingarten, Petra Lutter, Andreas Wattenberg, Martin Blueggel, Sonja Bailey, Joachim Klose et al.

    Pages 155-173

14. Isolation and Expansion of Tumor-Reactive Cytotoxic T-Cell Clones for Adoptive Immunotherapy

    • Helga Bernhard, Burkhard Schmidt, Dirk H. Busch, Christian Peschel

    Pages 175-184

15. Tracking Adoptively Transferred Antigen-Specific T-Cells With Peptide/MHC Multimers

    • Norbert Meidenbauer, Andreas Mackensen

    Pages 185-200

16. Gene Transfer of MHC-Restricted Receptors

    • Helmut W. H. G. Kessels, Monika C. Wolkers, Ton N. M. Schumacher

    Pages 201-213

17. Immunotherapy With CTL Restricted by Nonself MHC

    • Liquan Gao, Anne-Marie Downs, Hans J.

    Pages 215-228

18. Designing TCR for Cancer Immunotherapy

    • Ralf-Holger Voss, Jürgen Kuball, Matthias Theobald

    Pages 229-256

19. Isolation and Expansion of Tumor-Specific CD4+T-Cells by Means of Cytokine Secretion

    • Christian Becker

    Pages 257-264

20. Methods for the Ex Vivo Characterization of Human CD8+T Subsets Based on Gene Expression and Replicative History Analysis

    • Nathalie Rufer, Patrick Reichenbach, Pedro Romero

    Pages 265-283

An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

• Antigen
• autoimmunity
• cancer
• clinical trial
• cytokine
• immunotherapy
• infection
• leukemia
• proteins
• stem cell transplantation
• transplantation
• tumor

"...a comprehensive and authoritative collection of currently used protocols for adoptive immunotherapy in both pre-clinical and clinical settings." - Immunology News

"...will be not only useful for basic researchers but also clinical scientists aiming to translate basic research to a clinical setting." - Immunology and Cell Biology

• Research Department, Kantonsspital St. Gallen, Switzerland

  Burkhard Ludewig

• Department of Abdominal and Transplant Surgery, Hannover Medical School, Hannover, Germany

  Matthias W. Hoffmann

Policies and ethics