Abstract
Recombinant adenoviral (rAd) vectors are highly suitable for efficient genetic modification of dendritic cells (DC). In certain cases, the high immunogenicity of rAd may be a disadvantage This chapter describes the essential aspects of optimal rAd-mediated gene transfer into DC, discusses the consequences of the immunogenicity of rAd vectors, and provides suggestions to minimize these complications.
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References
Banchereau, J., Briere, F., Caux, C., et al. (2000) Immunobiology of dendritic cells. Annu. Rev. Immunol. 18, 767–811.
Russell, W. C. (2000) Update on adenovirus and its vectors. J. Gen. Virol. 81, 2573–604.
St. George, J. A. (2003) Gene therapy progress and prospects: adenoviral vectors. Gene Ther. 10, 1135–1141.
Rea, D., Schagen, F. H., Hoeben, R. C., et al. (1999) Adenoviruses activate human dendritic cells without polarization toward a T-helper type 1-inducing subset. J. Virol. 73, 10,245–10,253.
Nevins, J. R., Imperiale, M. J., Kao, H. T., Strickland, S., and Feldman, L. T. (1984) Detection of an adenovirus E1A-like activity in mammalian cells. Curr. Top. Microbiol. Immunol. 113, 15–19.
Amalfitano, A., Hauser, M. A., Hu, H., Serra, D., Begy, C. R., and Chamberlain, J. S. (1998) Production and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deleted. J. Virol. 72, 926–933.
Nyberg-Hoffman, C., Shabram, P., Li, W., Giroux, D., and Aguilar-Cordova, E. (1997) Sensitivity and reproducibility in adenoviral infectious titer determination. Nat. Med. 3, 808–811.
Fallaux, F. J., Bout, A., van der Velde, I., et al. (1998) New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses. Hum. Gene Ther. 9, 1909–1917.
Fallaux, F. J., Kranenburg, O., Cramer, S. J., et al. (1996) Characterization of 911: a new helper cell line for the titration and propagation of early region 1-deleted adenoviral vectors. Hum. Gene Ther. 7, 215–222.
Graham, F. L., Smiley, J., Russell, W. C., and Nairn, R. (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59–74.
Schagen, F. H., Rademaker, H. J., Rabelink, M. J., et al. (2000) Ammonium sulphate precipitation of recombinant adenovirus from culture medium: an easy method to increase the total virus yield. Gene Ther. 7, 1570–1574.
Rea, D., Havenga, M. J., van Den Assem, M., et al. (2001) Highly efficient transduction of human monocyte-derived dendritic cells with subgroup B fiber-modified adenovirus vectors enhances transgene-encoded antigen presentation to cytotoxic T cells. J. Immunol. 166, 5236–5244.
Belousova, N., Korokhov, N., Krendelshchikova, V., et al. (2003) Genetically targeted adenovirus vector directed to CD40-expressing cells. J. Virol. 77, 11,367–11,377.
Fontana, L., Nuzzo, M., Urbanelli, L., and Monaci, P. (2003) General strategy for broadening adenovirus tropism. J. Virol. 77, 11,094–11,104.
Gaggar, A., Shayakhmetov, D. M., and Lieber, A. (2003) CD46 is a cellular receptor for group B adenoviruses. Nat. Med. 9, 1408–1412.
Okada, N., Masunaga, Y., Okada, Y., et al. (2003) Dendritic cells transduced with gp100 gene by RGD fiber-mutant adenovirus vectors are highly efficacious in generating antiB16BL6 melanoma immunity in mice. Gene Ther. 10, 1891–1902.
Vogels, R., Zuijdgeest, D., van Rijnsoever, R., et al. (2003) Replication-deficient human adenovirus type 35 vectors for gene transfer and vaccination: efficient human cell infection and bypass of preexisting adenovirus immunity. J Virol. 77, 8263–8271.
de Gruijl, T. D., Luykx-de Bakker, S. A., Tillman, B. W., et al. (2002) Prolonged maturation and enhanced transduction of dendritic cells migrated from human skin explants after in situ delivery of CD40-targeted adenoviral vectors. J. Immunol. 169, 5322–5331.
Sallusto, F. and Lanzavecchia, A. (2000) Understanding dendritic cell and T-lymphocyte traffic through the analysis of chemokine receptor expression. Immunol. Rev. 177, 134–140.
Watanabe, S., Kagamu, H., Yoshizawa, H., et al. (2003) The duration of signaling through CD40 directs biological ability of dendritic cells to induce antitumor immunity. J. Immunol. 171, 5828–5836.
Langenkamp, A., Messi, M., Lanzavecchia, A., and Sallusto, F. (2000) Kinetics of dendritic cell activation: impact on priming of TH1, TH2 and nonpolarized T cells. Nat. Immunol. 1,311–316.
Kaisho, T. and Akira, S. (2002) Toll-like receptors as adjuvant receptors. Biochim. Biophys. Acta 1589, 1–13.
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Offringa, R., Kwappenberg, K., Rabelink, M., Rea, D., Hoeben, R. (2005). Adenoviral Transduction of Dendritic Cells. In: Ludewig, B., Hoffmann, M.W. (eds) Adoptive Immunotherapy: Methods and Protocols. Methods in Molecular Medicine™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-862-5:083
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DOI: https://doi.org/10.1385/1-59259-862-5:083
Publisher Name: Humana Press
Print ISBN: 978-1-58829-406-7
Online ISBN: 978-1-59259-862-5
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