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Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection

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Capillary Electrophoresis of Proteins and Peptides

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 276))

Abstract

The recently developed whole-column-imaging detection technique for capillary electrophoresis (CE) and capillary isoelectric focusing (CIEF), a commercial whole-column-imaged CIEF instrument and its standard operation protocol are introduced. Furthermore, new developments and applications of whole-column-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.

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Author information

Authors and Affiliations

  1. Convergent Bioscience Ltd., Toronto, Ontario, Canada

    Jiaqi Wu

  2. Department of Materials Science and Engineering, Fukui University, Fukui-shi, Japan

    Xing-Zheng Wu

  3. Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada

    Tiemin Huang & Janusz Pawliszyn

Authors
  1. Jiaqi Wu
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  2. Xing-Zheng Wu
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  3. Tiemin Huang
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  4. Janusz Pawliszyn
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Editor information

Editors and Affiliations

  1. Eli Lilly and Company, Indianapolis, IN

    Mark A. Strege

  2. Lilly Research Laboratories, Indianapolis, IN

    Avinash L. Lagu

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© 2004 Humana Press Inc.

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Wu, J., Wu, XZ., Huang, T., Pawliszyn, J. (2004). Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection. In: Strege, M.A., Lagu, A.L. (eds) Capillary Electrophoresis of Proteins and Peptides. Methods in Molecular Biology™, vol 276. Humana Press. https://doi.org/10.1385/1-59259-798-X:229

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  • DOI: https://doi.org/10.1385/1-59259-798-X:229

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-017-5

  • Online ISBN: 978-1-59259-798-7

  • eBook Packages: Springer Protocols

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