Capillary Electrophoresis of Proteins and Peptides

  • Mark A. Strege
  • Avinash L. Lagu

Part of the Methods in Molecular Biology™ book series (MIMB, volume 276)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Charles A. Lucy, Nicole E. Baryla, Ken K.-C. Yeung
    Pages 1-14
  3. David L. Good, Stacey Cummins-Bitz, Raeann M. Fields, Brian K. Nunnally
    Pages 121-136
  4. Maryam Azad, John Kaddis, Valerie Villareal, Lili Hernandez, Catherine Silverio, Frank A. Gomez
    Pages 153-168
  5. Jiaqi Wu, Xing-Zheng Wu, Tiemin Huang, Janusz Pawliszyn
    Pages 229-252
  6. Suzana Martinović, Ljiljana Paša-Tolić, Richard D. Smith
    Pages 291-304
  7. Back Matter
    Pages 325-332

About this book

Introduction

Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development.

Editors and affiliations

  • Mark A. Strege
    • 1
  • Avinash L. Lagu
    • 2
  1. 1.Eli Lilly and CompanyIndianapolis
  2. 2.Lilly Research LaboratoriesIndianapolis

Bibliographic information

  • DOI https://doi.org/10.1385/159259798X
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-017-5
  • Online ISBN 978-1-59259-798-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Chemical Manufacturing
Biotechnology
Consumer Packaged Goods
Pharma