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Recombinant Protein Production in Yeasts

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Recombinant Gene Expression

Part of the book series: Methods in Molecular Biology ((MIMB,volume 267))

Abstract

Recombinant DNA (rDNA) technologies (genetic, protein, and metabolic engineering) allow the production of a wide range of peptides, proteins, and biochemicals from naturally nonproducing cells. This technology, now approx 25 yr old, is becoming one of the most important technologies developed in the 20th century.

Pharmaceutical products and industrial enzymes were the first biotech products on the world market made by means of rDNA. Despite important advances regarding rDNA applications in mammalian cells, yeasts still represent attractive hosts for the production of heterologous proteins. In this review we summarize the advantages and limitations of the main and promising yeast hosts.

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Author information

Authors and Affiliations

  1. Department of Biotechnology and Bioscience, University of Milano-Bicocca, Milano, Italia

    Danilo Porro

  2. Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Vienna, Austria

    Diethard Mattanovich

Authors
  1. Danilo Porro
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  2. Diethard Mattanovich
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Editor information

Editors and Affiliations

  1. Centro de Investigación en Biotecnología, UAEM, Cuernavaca, Morelos, México

    Paulina Balbás

  2. Virginia Polytechnic Institute and State University, Blacksburg, VA

    Argelia Lorence

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© 2004 Humana Press Inc., Totowa, NJ

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Porro, D., Mattanovich, D. (2004). Recombinant Protein Production in Yeasts. In: Balbás, P., Lorence, A. (eds) Recombinant Gene Expression. Methods in Molecular Biology, vol 267. Humana Press. https://doi.org/10.1385/1-59259-774-2:241

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  • DOI: https://doi.org/10.1385/1-59259-774-2:241

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-262-9

  • Online ISBN: 978-1-59259-774-1

  • eBook Packages: Springer Protocols

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