Recombinant Gene Expression

Reviews and Protocols

  • Paulina Balbás
  • Argelia Lorence

Part of the Methods in Molecular Biology book series (MIMB, volume 267)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. General Issues About Recombinant Gene Expression

    1. Front Matter
      Pages 1-1
    2. Laura A. Palomares, Sandino Estrada-Moncada, Octavio T. Ramírez
      Pages 15-51
  3. Prokaryotes

    1. Front Matter
      Pages 75-75
    2. Paulina Balbás, Francisco Bolívar
      Pages 77-90
    3. Jean-Claude Boulain, Frédéric Ducancel
      Pages 101-112
    4. Fernando Valle, Noemí Flores
      Pages 113-122
    5. Sylvie Le Borgne, Francisco Bolívar, Guillermo Gosset
      Pages 135-143
    6. Jadwiga Wild, Waclaw Szybalski
      Pages 145-154
    7. Jadwiga Wild, Waclaw Szybalski
      Pages 155-167
    8. James R. Swartz, Michael C. Jewett, Kim A. Woodrow
      Pages 169-182
    9. Amalia S. Afendra, Carmen Vargas, Joaquín J. Nieto, Constantin Drainas
      Pages 209-223
    10. Angela Duilio, Maria Luisa Tutino, Gennaro Marino
      Pages 225-237
  4. Fungi

    1. Front Matter
      Pages 239-239
    2. Danilo Porro, Diethard Mattanovich
      Pages 241-258

About this book


In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of nonprotein molecules. The readily reproducible protocols offer new host-vector systems, such as Antarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. There are also novel heterologous gene expression schemes for cell-free systems, plant chloroplasts, microplates, hairy roots, and animal milk, and new developments in consolidated heterologous systems. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and forward looking, Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field not only the understanding, but also the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Editors and affiliations

  • Paulina Balbás
    • 1
  • Argelia Lorence
    • 2
  1. 1.Centro de Investigación en BiotecnologíaUAEMCuernavacaMéxico
  2. 2.Virginia Polytechnic Institute and State UniversityBlacksburg

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-262-9
  • Online ISBN 978-1-59259-774-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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