Abstract
This chapter describes methods for the production and use of antibody microarrays. The methods are divided into (a) antibody handling and microarray production, (b) sample preparation, and (c) microarray use. Two types of detection methods are described: direct labeling and a fluorescence-linked immunosorbent assay (FLISA). In the direct labeling method, all proteins in a complex mixture are labeled with either a fluorophore or a hapten that allows subsequent detection. In FLISA detection, a capture antibody on the microarray captures the unlabeled protein target, which is detected by a detection antibody and a fluorophore-labeled secondary antibody. Each method has particular optimal uses, which are discussed in the text.
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© 2004 Humana Press Inc., Totowa, NJ
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Haab, B.B., Zhou, H. (2004). Multiplexed Protein Analysis Using Spotted Antibody Microarrays. In: Fung, E.T. (eds) Protein Arrays. Methods in Molecular Biology, vol 264. Humana Press. https://doi.org/10.1385/1-59259-759-9:033
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DOI: https://doi.org/10.1385/1-59259-759-9:033
Publisher Name: Humana Press
Print ISBN: 978-1-58829-255-1
Online ISBN: 978-1-59259-759-8
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