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Protein Arrays

Methods and Protocols

  • Eric T. Fung

Part of the Methods in Molecular Biology book series (MIMB, volume 264)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Hendrik Weiner, Thomas Faupel, Konrad Büssow
    Pages 1-13
  3. Michele Gilbert, Todd C. Edwards, Joanna S. Albala
    Pages 15-23
  4. Alexei Kurakin, Susan Wu, Dale E. Bredesen
    Pages 47-60
  5. Petra Pavlickova, Hubert Hug
    Pages 73-83
  6. Rina Y. P. Lue, Grace Y. J. Chen, Qing Zhu, Marie-Laure Lesaicherre, Shao Q. Yao
    Pages 85-100
  7. Helen Petach, Rachel Ostroff, Chad Greef, Gregory M. Husar
    Pages 101-110
  8. Susan M. Varnum, Ronald L. Woodbury, Richard C. Zangar
    Pages 161-172
  9. Alexsandra Espejo, Mark T. Bedford
    Pages 173-181
  10. Sangpen Chamnongpol, Xianqiang Li
    Pages 183-189
  11. Mahesh Uttamchandani, Grace Y. J. Chen, Marie-Laure Lesaicherre, Shao Q. Yao
    Pages 191-204
  12. Vanitha Thulasiraman, Zheng Wang, Anjali Katrekar, Lee Lomas, Tai-Tung Yip
    Pages 205-214
  13. Ruo-Pan Huang
    Pages 215-231
  14. Ye Fang, Brian Webb, Yulong Hong, Ann Ferrie, Fang Lai, Anthony G. Frutos et al.
    Pages 233-243
  15. Lisa E. Bradbury, James F. LeBlanc, Diane B. McCarthy
    Pages 245-257
  16. Kate Gilbert, Sharel Figueredo, Xiao-Ying Meng, Christine Yip, Eric T. Fung
    Pages 259-269
  17. Tito Bacarese-Hamilton, Andrea Ardizzoni, Julian Gray, Andrea Crisanti
    Pages 271-283
  18. Back Matter
    Pages 281-281

About this book

Introduction

Protein arrays make possible the detection and quantitation of many proteins simultaneously, thus enabling researchers to ask fundamental questions about biological processes and to discover biomarkers that can be used diagnostically. In Protein Arrays: Methods and Protocols, innovative experimentalists describe in detail the methods they have developed to synthesize and construct protein arrays for basic and clinical research. The authors present protocols to create and immobilize the capture substrate-the first task in designing a protein array-using a variety of affinity capture reagents, including antibodies, peptides, aptamers, biotin, chemical reagents, and chromatographic substrates. Once synthesized, these arrays can be used to analyze protein-protein interactions and posttranslational modifications, such as phosphorylation, as well as to discover and characterize potential diagnostic markers. Protocols to accomplish these tasks are also presented. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Diverse and highly practical, Protein Arrays: Methods and Protocols offers basic and clinical investigators a broad spectrum of approaches to the generation of protein arrays, as well as their uses in biomarkers discovery, in assay development, in clinical sample testing, in signal transduction analysis and characterization, and for creating the next generation of molecular tools.

Editors and affiliations

  • Eric T. Fung
    • 1
  1. 1.Ciphergen Biosystems Inc.Fremont

Bibliographic information

  • DOI https://doi.org/10.1385/1592597599
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-255-1
  • Online ISBN 978-1-59259-759-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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