Abstract
During the developmental process of T cells in the thymus, immature CD4CD8 double positive thymocytes (DP) are subject to undergo positive selection process to become either CD4 single positive (SP) or CD8-SP mature cells (1–3). Since both CD4-SP and CD8-SP mature T cells are the progeny of immature DP thymocytes, positive selection requires a lineage commitment step in which synthesis of one or the other coreceptor molecule is terminated. The mechanism of CD4/8 lineage commitment process, i.e., how does thymocyte stop one or the other coreceptor synthesis, is still unclear although many types of models have been presented (4–10).
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© 2000 Humana Press Inc., Totowa, NJ
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Suzuki, H. (2000). Analysis of CD4/CD8 Lineage Commitment by Pronase Treatment and Reexpression Assay. In: Kearse, K.P. (eds) T Cell Protocols. Methods in Molecular Biology™, vol 134. Humana Press. https://doi.org/10.1385/1-59259-682-7:63
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DOI: https://doi.org/10.1385/1-59259-682-7:63
Publisher Name: Humana Press
Print ISBN: 978-0-89603-810-3
Online ISBN: 978-1-59259-682-9
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