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  • Book
  • © 2000

T Cell Protocols

Development and Activation

Editors:

  1. Kelly P. Kearse

    1. Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 134)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Overview: For Chapters 2 – 15

    1. Front Matter

      Pages 1-1
      PDF

    2. Insights into T-Cell Development Using Transgenic and Knockout Mice

      • M. Albert. Basson, Rose Zamoyska
      Pages 3-22

  3. T Cell Development

    1. Front Matter

      Pages 23-23
      PDF

    5. Transfection and Transcription of Genes in Developing Thymocytes

      • Alison M. Michie, Juan Carlos Zúñiga-Pflücker
      Pages 55-62

    7. Intrathymic Delivery of MHC Genes Using Recombinant Adenoviruses

      • Ronald Rooke, Christophe Benoist, Diane Mathis
      Pages 69-79

    8. Quantitative Analysis of the Usage of Human T-Cell Receptor α and β Chain Variable Regions by Reverse Dot Blot Hybridization

      • Takaji Matsutani, Takeshi Yoshioka, Yuji Tsuruta, Shoji Iwagami, Tomoko Toyosaki-Maeda, Ryuji Suzuki
      Pages 81-101

    10. Assessing Apoptosis of Developing T Cells by Flow Cytometry

      • Caroline D. V. A. Bishop, Amy Jost, Elizabeth Crane, Jennifer A. Punt
      Pages 117-131

    11. Isolation of T-Cell Subsets by Magnetic Cell Sorting (MACS)

      • Luminita A. Stanciu, Ratko Djukanović
      Pages 133-141

    12. Isolation of T-Cell Antigens by Retrovirus-Mediated Expression Cloning

      • Toshio Kitamura, Yoshihiro Morikawa
      Pages 143-152

    13. Flow Cytometric Analysis of Murine T Lymphocytes

      • Melanie S. Vacchio, Elizabeth W. Shores
      Pages 153-176

    14. Production and Characterization of Immature Murine T-Lymphoma Cell Lines

      • Ellen R. Richie, Dawn A. Walker
      Pages 177-184

    15. Production and Characterization of T-Cell Hybridomas

      • Janice White, John Kappler, Philippa Marrack
      Pages 185-193

  4. Overview for Chapters 17–29

    1. Front Matter

      Pages 195-195
      PDF
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About this book

The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology. Invariably, in putting together a book like this it is difficult to decide which methods to include and which to leave out. To this end methods were selected from a variety of disciplines, including cellular immunology, b- chemistry, and molecular biology, to try to provide something of interest for everyone who works on T-cell development and activation. I would like to mention that my primary reason for agreeing to put this book together is that, when I was a graduate student, I purchased a copy of Selected Methods in Cellular Immunology by Mishell and Shigii which proved a tremendous help in learning the basics of one-and two- dimensional gel te- niques (and other methods). The cover has long since fallen off, but it still remains one of my most valued reference books for the laboratory. It is my hope that T Cell Protocols: Development and Activation will prove similarly useful to current and future scientists wishing to learn new methods for expl- ing the development and activation of T cells.
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Reviews

"...The protocols themselves are highly detailed, and also refer to a notes section in each chapter which is used to impart tips and tricks which are usually essential to getting a technique to work well, but which do not often come over in the usual material and methods section of papers. Each of the chapters is also well referenced. The book would be invaluable for establishing a particular technique or set of techniques in an environment where there was little or no previous experience of such as as teaching aid for when new research staff or students come into a laboratory."- The Journal of Infection
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Editors and Affiliations

  • Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville

    Kelly P. Kearse

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Bibliographic Information

  • Book Title: T Cell Protocols

  • Book Subtitle: Development and Activation

  • Editors: Kelly P. Kearse

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596827

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2000

  • eBook ISBN: 978-1-59259-682-9Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 365

  • Number of Illustrations: 267 b/w illustrations

  • Topics: Immunology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

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