Abstract
The size and shape of an individual T lymphocyte can reflect activation or functional status. Therefore, information from a visual assessment of morphology can provide important criteria for determining extrinsic factors that regulate the physiological responses of lymphocytes. The combination of video microscopy and digital imaging can detect and quantify differences in cellular morphology, and therefore, it has been exploited and developed into a reproducible, sensitive method for the direct study of lymphocyte behavior. We have used this method to quantitate human T-lymphocyte coactivation, leading to cellular blastogenesis and proliferation (1), the induction and kinetics of lymphocyte aggregation (2), and lymphocyte adhesion and the formation of pseudopodia (3).
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References
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© 2000 Humana Press Inc., Totowa, NJ
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McIntyre, B.W., Teague, T.K., Woodside, D.G., Wooten, D.K. (2000). Digital Image Analysis of Lymphocyte Activation. In: Kearse, K.P. (eds) T Cell Protocols. Methods in Molecular Biology™, vol 134. Humana Press. https://doi.org/10.1385/1-59259-682-7:257
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DOI: https://doi.org/10.1385/1-59259-682-7:257
Publisher Name: Humana Press
Print ISBN: 978-0-89603-810-3
Online ISBN: 978-1-59259-682-9
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