Abstract
The profile of the two-dimensional (2-D) separation of the protein complement (proteome) of eukaryotic cells and tissues typically contains obvious “trains” of spots that differ in pI and/or apparent molecular mass. These are usually isoforms of the same protein and result from a variety of posttranslational modifications. There is growing evidence that alterations to the glycosylation of a protein can be correlated with developmental and pathological changes; these changes can be visualized on the 2-D separation by alterations in the pattern of these glycoprotein isoforms.
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© 1999 Humana Press Inc., Totowa, NJ
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Packer, N.H., Ball, M.S., Devine, P.L. (1999). Glycoprotein Detection of 2-D Separated Proteins. In: Link, A.J. (eds) 2-D Proteome Analysis Protocols. Methods in Molecular Biology, vol 112. Humana Press. https://doi.org/10.1385/1-59259-584-7:341
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DOI: https://doi.org/10.1385/1-59259-584-7:341
Publisher Name: Humana Press
Print ISBN: 978-0-89603-524-9
Online ISBN: 978-1-59259-584-6
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