2-D Proteome Analysis Protocols

  • Andrew J. Link

Part of the Methods in Molecular Biology book series (MIMB, volume 112)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Jenny Fichmann, Reiner Westermeier
    Pages 1-7
  3. Ruth A. VanBogelen, Frederick C. Neidhardt
    Pages 21-29
  4. David M. Schieltz
    Pages 31-34
  5. Melinda L. Ramsby, Gregory S. Makowski
    Pages 53-66
  6. Jean-Charles Sanchez, Denis F. Hochstrasser
    Pages 87-93
  7. Akira Tsugita, Masaharu Kamo
    Pages 95-97
  8. Andrew J. Link, Nick Bizios
    Pages 105-107
  9. Joachim Klose
    Pages 147-172
  10. Jenny Fichmann
    Pages 173-174

About this book

Introduction

In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on a 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. The book is a perfect complement to the genome sequencing project for answering biological questions.

2-D Gel Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions. Whether it is a question of global protein analysis or evaluating a cell's response to internal or external stimuli, the advanced methods described here will enable today's researchers better to understand how cells work and open new possibilities for drug discovery.

Editors and affiliations

  • Andrew J. Link
    • 1
  1. 1.Department of Molecular BiotechnologyUniversity of WashingtonSeattle

Bibliographic information

  • DOI https://doi.org/10.1385/1592595847
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-524-9
  • Online ISBN 978-1-59259-584-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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