Abstract
Mammalian acidic lipases belong to a family of lipases that share the ability to maintain activity under acidic conditions. This family includes pre-duodenal lipases and lysosomal lipase and shows no sequence homology with other known lipase families (1,2). Human gastric lipase (glycerol ester hydrolase, EC 3.1.1.3, HGL) is secreted by chief cells of the fundic part of the stomach (3) where it initiates the digestion of triacylglycerols (4). Human lysosomal acid lipase (HLAL , EC 3.1.1.13) hydrolyzes cholesteryl esters and triglycerides that are delivered to the lysosomes by low density lipoprotein receptor-mediated endocytosis (5).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Petersen, S. B. and Drabløs, F. (1994) A sequence analysis of lipases, esterases and related proteins in, Lipases: Their biochemistry, structure and application, (Woolley P. and Petersen S., eds.), Cambridge University Press, UK, pp. 23–48.
Aoubala, M., Carrière, F., Ransac, S., Verger, R., and de Caro, A. (1995) Nouveaux regards sur les lipases digestives. Regards sur la Biochimie 2, 13–22.
Moreau, H., Laugier, R., Gargouri, Y., Ferrato, F., and Verger, R. (1988) Human preduodenal lipase is entirely of gastric fundic origin. Gastroenterology 95, 1221–1226.
Carrière, F., Barrowman, J. A., Verger, R., and Laugier, R. (1993) Secretion and contribution to lipolysis of gastric and pancreatic lipases during a test meal in humans. Gastroenterology 105, 876–888.
Goldstein, J. L., Dana, S. E., Faust, J. R., Beaudet, A.L., and Brown, M. S. (1975) Role of lysosomal acid lipase in the metabolism of plasma low density lipoproteins. J. Biol. Chem. 250, 8487–8795.
Maiorella, B., Inlow, D., Shauger, A., and Harano, D. (1988) Large scale insect cell culture for recombinant protein production. Biotechnology 6, 1406–1410.
Caron, A.W., Archambault, L. and Massie, B. (1990) High level recombinant protein production in bioreactors using the baculovirus-insect cell expression system. Biotechnol. Bioeng. 36, 1133–1140.
Sheriff, S., Du, H., and Grabowski, G. A. (1995) Characterization of lysosomal acid lipase by site-directed mutagenesis and heterologous expression. J. Biol. Chem. 270, 27,766–27,772.
Luckow, V. A. and Summers, M. D. (1988) Trends in the development of baculovirus expression vectors. Biotechnology 6, 47–55.
Aeed, P. A. and Elhammer, A. P. (1994) Glycosylation of recombinant prorenin in insect cells: the insect cell line Sf9 does not express the mannose 6-phosphate recognition signal. Biochemistry 33, 8793–8797.
von Heijne, G. (1986) A new method for predicting signal sequence cleavage sites. Nucl. Ac. Res. 14, 4683–4690.
Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467.
Anderson, R. A. and Sando, G. N. (1991) Cloning and expression of cDNA encoding human lysosomal acid lipase/cholesteryl ester hydrolase. Similitaries to gastric and lingual lipases. J. Biol. Chem. 266, 22,479–22,484.
Ameis, D., Merkel, M., Eckerskorn, C, and Greten, H. (1994) Purification, characterization and molecular cloning of human hepatic lysosomal acid lipase. Eur. J Biochem. 219, 905–914.
Gargouri, Y., Piéroni, G., Rivière, C, Saunière, J.-F., Lowe, P. A., Sarda, L., and Verger, R. (1986) Kinetic assay of human gastric lipase on short-and long-chain triacylglycerol emulsions. Gastroenterology 91, 919–925.
Wicker-Planquart, C, Canaan, S., Riviere, M., Dupuis, L., and Verger, R. (1996) Expression in insect cells and purification of a catalytically active recombinant human gastric lipase. Protein Eng. 9, 1225–1232.
Negre, A. E., Salvayre, R. S., Dagan, A., and Gatt, S. (1985) New fluorometric assay of lysosomal lipase and its application to the diagnosis of Wolman and cholesteryl ester storage diseases. Clinica Chimica Acta 149, 81–88.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc, Totowa, NJ
About this protocol
Cite this protocol
Canaan, S., Dupuis, L., Rivière, M., Verger, R., Wicker-Planquart, C. (1999). Modulation of the Expression Level of Human Acidic Lipases by Various Signal Peptides. In: Doolittle, M., Reue, K. (eds) Lipase and Phospholipase Protocols. Methods in Molecular Biology™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-581-2:203
Download citation
DOI: https://doi.org/10.1385/1-59259-581-2:203
Publisher Name: Humana Press
Print ISBN: 978-0-89603-546-1
Online ISBN: 978-1-59259-581-5
eBook Packages: Springer Protocols