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Lipase and Phospholipase Protocols

  • Mark Doolittle
  • Karen Reue

Part of the Methods in Molecular Biology™ book series (MIMB, volume 109)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Lipase Activity, Purification, and Expression

    1. Front Matter
      Pages N1-N1
    2. Wonhwa Cho, Shih-Kwang Wu, Edward Yoon., Lenka Lichtenbergova
      Pages 7-17
    3. Wonhwa Cho, Sang Kyou Han, Byung-In Lee, Yana Snitko, Rajiv Dua
      Pages 31-38
    4. Akhlaq A. Farooqui, Hsiu-Chiung Yang, Yutaka Hirashima, Lloyd A. Horrocks
      Pages 39-47
    5. Diana M. Stafforini, Larry W. Tjoelker
      Pages 49-58
    6. Chi-Sun Wang, Azar Dashti, Deborah Downs
      Pages 71-79
    7. Véronique Briquet-Laugier, Osnat Ben-Zeev, Mark H. Doolittle
      Pages 81-94
    8. Martin Merkel, Anne-Christine Tilkorn, Heiner Greten, Detlev Ameis
      Pages 95-107
    9. Cecilia Holm, Torben Østerlund
      Pages 109-121
    10. John S. Parks, Abraham K. Gebre, James W. Furbee
      Pages 123-131
    11. Akhlaq A. Farooqui, Lloyd A. Horrocks
      Pages 133-143
    12. André Bensadoun, Jean Hsu, Barry Hughes
      Pages 145-150
    13. André Bensadoun, Barry Hughes, Kristan Melford, Jean Hsu, Dawn L. Braesaemle
      Pages 151-156
    14. Cecilia Holm, Juan Antonio Contreras
      Pages 165-175
    15. Anne-Christine Tilkorn, Martin Merkel, Heiner Greten, Detlev Ameis
      Pages 177-185
    16. Sofiane Bezzine, Francine Ferrato, Véronique Lopez, Alain de Caro, Robert Verger, Frédéric Carrière
      Pages 187-202
    17. Stéphane Canaan, Liliane Dupuis, Mireille Rivière, Robert Verger, Catherine Wicker-Planquart
      Pages 203-213
  3. Biochemical Characterization

    1. Front Matter
      Pages N3-N3
    2. Alain De Caro, Sofiane Bezzine, Véronique Lopez, Mustapha Aoubala, Cécile Daniel, Robert Verger et al.
      Pages 239-256
    3. Osnat Ben-Zeev, Mark H. Doolittle
      Pages 257-266
    4. Sivaram Pillarisetti
      Pages 267-278
    5. Stéphane Ransac, Margarita Ivanova, Ivan Panaiotov, Robert Verger
      Pages 279-302
    6. Wonhwa Cho, Zhen Shen
      Pages 303-307
  4. Molecular Genetic Characterization

    1. Front Matter
      Pages N5-N5
    2. Gouri Ranganathan, Philip A. Kern
      Pages 329-335
    3. Gouri Ranganathan, Philip A. Kern
      Pages 337-341
    4. Clay F. Semenkovich
      Pages 343-355
  5. Back Matter
    Pages 357-362

About this book

Introduction

Lipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for the determination of lipolytic activity, proven strategies for the expression and purification of recombinant lipases and phospholipases, and the characterization of lipases by such techniques as immunodetection, subunit size determination, lipase kinetics, and ligand interactions. In addition, there are methods for the molecular and genetic analysis of the lipases, the quantification of mRNA levels, and in vitro-coupled transcription/translation. The protocols cover more than a dozen specific enzymes, and many can be applied directly to all lipases and phospholipases.

Lipase and Phospholipase Protocols constitutes a highly useful single-volume handbook of basic procedures and strategies to investigate this unique class of enzymes. Its step-by-step format works perfectly at the laboratory bench or by the computer, providing practical information and examples that will lead to successful design and execution of specific techniques. It not only furnishes a technical framework for the investigation of known lipases and phospholipases, but provides well-established methods readily daptable to the discovery of new lipase enzymes.



Editors and affiliations

  • Mark Doolittle
    • 1
  • Karen Reue
    • 1
  1. 1.University of California and West Los Angeles VA Medical CenterLos Angeles

Bibliographic information

  • DOI https://doi.org/10.1385/1592595812
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-546-1
  • Online ISBN 978-1-59259-581-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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