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One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells

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Lipase and Phospholipase Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 109))

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Abstract

The baculovirus expression system is very convenient to produce recombinant pancreatic lipases and mutants thereof, in substantial amounts (10–50 mg of enzyme per liter of culture) for structure-function studies (see also Chapters 17, 18, and 20 in this volume). Using the naturally occurring leader sequence, recombinant enzymes are secreted by insect cells and are easy to purify in a one-step procedure when insect cells are grown in a serum-free medium.

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Authors and Affiliations

  1. Laboratoire de Lipolyse Enzymatique, CNRS, Marseille, France

    Sofiane Bezzine, Francine Ferrato, Véronique Lopez, Alain de Caro, Robert Verger & Frédéric Carrière

Authors
  1. Sofiane Bezzine
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  2. Francine Ferrato
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  3. Véronique Lopez
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  4. Alain de Caro
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  5. Robert Verger
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  6. Frédéric Carrière
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Editor information

Editors and Affiliations

  1. University of California and West Los Angeles VA Medical Center, Los Angeles, CA

    Mark Doolittle  & Karen Reue  & 

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© 1999 Humana Press Inc, Totowa, NJ

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Bezzine, S., Ferrato, F., Lopez, V., de Caro, A., Verger, R., Carrière, F. (1999). One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells. In: Doolittle, M., Reue, K. (eds) Lipase and Phospholipase Protocols. Methods in Molecular Biology™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-581-2:187

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  • DOI: https://doi.org/10.1385/1-59259-581-2:187

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-546-1

  • Online ISBN: 978-1-59259-581-5

  • eBook Packages: Springer Protocols

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