Abstract
Dictyostelium discoideum are simple eukaryotic amoebas that when starved of a food source have the remarkable ability to spontaneously aggregate and differentiate to form a cluster of spores on top of a stalk of vacuolated cells (see Fig. 1 and ref. 1). The process of aggregation, although quite fascinating to simply observe, has proven extremely powerful for the molecular dissection of chemotaxis signaling pathways. Mechanisms of chemoattractant sensing discovered in D. discoideum have been tested in higher eukaryotic systems and were found to be strikingly similar (2). In particular, a paradigm for directional sensing has been derived from studies in D. discoideum (3).
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References
Parent, C. A. and Devreotes, P. N. (1996) Molecular genetics of signal transduction in Dictyostelium. Annu. Rev. Biochem. 65, 411–440.
Devreotes, P. N. and Zigmond, S. H. (1988) Chemotaxis in eukaryotic cells: a focus on leukocytes and Dictyostelium, Annu. Rev. Cell Biol. 4, 649–686.
Parent, C. A. and Devreotes, P. N. (1999) A cell’s sense of direction. Science 284, 765–770.
Hadwiger, J. A., Lee, S., and Firtel, R. A. (1994) The G alpha subunit G alpha 4 couples to pterin receptors and identifies a signaling pathway that is essential for multicellular development in Dictyostelium, Proc. Natl. Acad. Sci. USA 91, 10,566–10,570.
Xiao, Z., Zhang, N., Murphy, D. B., and Devreotes, P. N. (1997) Dynamic distribution of chemoattractant receptors in living cells during chemotaxis and persistent stimulation. J. Cell Biol. 139, 365–374.
Jin, T., Zhang, N., Long, Y., Parent, C. A., and Devreotes, P. N. (2000) Localization of the G protein betagamma complex in living cells during chemotaxis. Science 287, 1034–1036.
Lilly, P. J. and Devreotes, P. N. (1995) Chemoattractant and GTP gamma S-mediated stimulation of adenylyl cyclase in Dictyostelium requires translocation of CRAC to membranes. J. Cell Biol. 129, 1659–1665.
Parent, C. A., Blacklock, B. J., Froehlich, W. M., Murphy, D. B., and Devreotes, P. N. (1998) G Protein signaling events are activated at the leading edge of chemotactic cells. Cell 95, 81–91.
Iijima, M. and Devreotes, P. (2002) Tumor suppressor PTEN mediates sensing of chemoattractant gradients. Cell 109, 599–610.
Funamoto, S., Meili, R., Lee, S., Parry, L., and Firtel, R. A. (2002) Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis. Cell 109, 611–623.
Rickert, P., Weiner, O. D., Wang, F., Bourne, H. R., and Servant, G. (2000) Leukocytes navigate by compass: roles of PI3Kgamma and its lipid products. Trends Cell Biol. 10, 466–473.
Wang, F., Herzmark, P., Weiner, O. D., Srinivasan, S., Servant, G., and Bourne, H. R. (2002) Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils. Nat. Cell Biol. 4, 513–518.
Weiner, O. D., Neilsen, P. O., Prestwich, G. D., Kirschner, M. W., Cantley, L. C., and Bourne, H. R. (2002) A PtdInsP(3)-and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity. Nat. Cell Biol. 4, 509–513.
Kuspa, A. and Loomis, W. F. (1992) Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc. Natl. Acad. Sci. USA 89, 8803–8807.
Robinson, D. N. and Spudich, J. A. (2000) Dynacortin, a genetic link between equatorial contractility and global shape control discovered by library complementation of a Dictyostelium discoideum cytokinesis mutant. J. Cell Biol. 150, 823–838.
van Es, S. and Devreotes, P. N. (1999) Molecular basis of localized responses during chemotaxis in amoebae and leukocytes. Cell. Mol. Life Sci. 55, 1341–1351.
Sutoh, K. (1993) A transformation vector for Dictyostelium discoideum with a new selectable marker bsr. Plasmid 30, 150–154.
Kuwayama, H., Obara, S., Morio, T., Katoh, M., Urushihara, H., and Tanaka, Y. (2002) PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. Nucleic Acids Res. 30, E2.
Blaauw, M., Linskens, M. H., and van Haastert, P. J. (2000) Efficient control of gene expression by a tetracycline-dependent transactivator in single Dictyostelium discoideum cells. Gene 252, 71–82.
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© 2004 Humana Press Inc., Totowa, NJ
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Landree, M.A., Devreotes, P.N. (2004). Analyzing Chemotaxis Using Dictyostelium discoideum as a Model System. In: D’Ambrosio, D., Sinigaglia, F. (eds) Cell Migration in Inflammation and Immunity. Methods in Molecular Biology™, vol 239. Humana Press. https://doi.org/10.1385/1-59259-435-2:91
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DOI: https://doi.org/10.1385/1-59259-435-2:91
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