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Cell Migration in Inflammation and Immunity

Methods and Protocols

  • Daniele D’Ambrosio
  • Francesco Sinigaglia

Part of the Methods in Molecular Biology™ book series (MIMB, volume 239)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Silvano Sozzani, Annunciata Vecchi, Paola Allavena, Alberto Mantovani
    Pages 1-15
  3. Paul Proost, Frank Mahieu, Evemie Schutyser, Jo Van Damme
    Pages 27-44
  4. Lucia Colantonio, Andrea Iellem, Daniele D’Ambrosio
    Pages 45-52
  5. Mark A. Landree, Peter N. Devreotes
    Pages 91-104
  6. Sergio A. Lira, Borna Mehrad, Shu-Cheng Chen, Petronio Zalamea, David J. Kinsley, Maria T. Wiekowski et al.
    Pages 105-122
  7. Claire L. Adams, Catherine M. Rush, Karen M. Smith, Paul Garside
    Pages 133-146
  8. Margherita Mariani, Paola Panina-Bordignon
    Pages 147-165
  9. Clare M. Lloyd, Jose-Carlos Gutierrez-Ramos
    Pages 199-209
  10. Adam P. Curnock, Yannis Sotsios, Stephen G. Ward
    Pages 211-221
  11. Giovanni Bernardini, Domenico Ribatti, Gaia Spinetti, Lucia Morbidelli, Marina Ziche, Angela Santoni et al.
    Pages 223-232
  12. Leonor Kremer, Gabriel Márquez
    Pages 243-260
  13. Back Matter
    Pages 269-277

About this book

Introduction

Cell migration is now well recognized as a critical component of the inflammatory disease process, so that its proper understanding promises to generate both ground-breaking basic discoveries and the development of novel therapeutics. In Cell Migration in Inflammation and Immunity: Methods and Protocols, leading cell biologists and immunologists present their most widely useful and innovative techniques for studying the molecular and cellular basis of this phenomenon. Describing each method in step-by-step detail, the authors provide a series of focused, cutting-edge techniques proceeding from the in vitro analysis of cell migration and the molecular mechanisms underlying this process, to methodologies for the analysis of cell migration in vivo. Methods for the analysis of rapid leukocyte adhesion under flow conditions in vitro are described, which may prove especially fruitful for scientists exploring the molecular mechanisms underlying both vascular recognition and leukocyte-endothelium interaction. Experimental approaches useful in establishing the role of cell migration in the pathogenesis of both acute and chronic inflammatory diseases are emphasized. Each fully tested protocol includes an introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and how to avoid known pitfalls.
Comprehensive and cutting-edge, Cell Migration in Inflammation and Immunity: Methods and Protocols offers novice and experienced investigators alike a collection of powerful techniques for studying the molecular basis and pathophysiological significance of cell migration in inflammatory and immune diseases, as well as for the development of novel therapeutics.

Editors and affiliations

  • Daniele D’Ambrosio
    • 1
  • Francesco Sinigaglia
    • 1
  1. 1.BioXell S.p.A.MilanItaly

Bibliographic information

  • DOI https://doi.org/10.1385/1592594352
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-102-8
  • Online ISBN 978-1-59259-435-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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