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Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I

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Membrane Protein Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 228))

Abstract

The mitochondrial carnitine palmitoyltransferase (CPT) system of enzymes permits the entry of long-chain acyl groups into the mitochondrial matrix, the site of β-oxidation. First conceptualized as a mechanism for mitochondrial fatty acid transport almost four decades ago (1,2), this multicomponent enzyme/transporter system is now recognized to play a primary role in the regulation of mitochondrial fatty acid oxidation and, hence, constitutes a critical control point in cellular lipid metabolism (3–5).

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Author information

Authors and Affiliations

  1. Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA

    Nicholas F. Brown

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  1. Nicholas F. Brown
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Editor information

Editors and Affiliations

  1. Department of Chemistry, Villanova University, Villanova, PA

    Barry S. Selinsky

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© 2003 Humana Press Inc., Totowa, NJ

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Brown, N.F. (2003). Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I. In: Selinsky, B.S. (eds) Membrane Protein Protocols. Methods in Molecular Biology, vol 228. Humana Press. https://doi.org/10.1385/1-59259-400-X:281

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  • DOI: https://doi.org/10.1385/1-59259-400-X:281

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-124-0

  • Online ISBN: 978-1-59259-400-9

  • eBook Packages: Springer Protocols

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