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Membrane Protein Protocols

Expression, Purification, and Characterization

  • Barry S. Selinsky
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 228)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Expression of Membrane Proteins

    1. Front Matter
      Pages 1-1
    2. Kaj Frank Jensen, Sine Larsen
      Pages 11-21
    3. Dirk Schneider, Christian L. Schmidt, Andreas Seidler
      Pages 37-47
    4. Chang-Hoon Han, Moritz Werder, Wilk von Gustedt, Helmut Hauser
      Pages 49-63
    5. Giel J. C. G. M. Bosman, Jenny van Oostrum, Githa Breikers, Petra H. M. Bovee-Geurts, Corne H. W. Klaassen, WiMem J. DeGrip
      Pages 73-86
  3. Detergent Selection in Membrane Protein Purification

    1. Front Matter
      Pages 87-87
    2. Robert Staudinger, Juan C. Bandrés
      Pages 103-109
  4. Membrane Protein Purification

    1. Front Matter
      Pages 129-129
    2. Christian Heinz, Eva Roth, Michael Niederweis
      Pages 139-150
    3. Benjamin L. Clarke
      Pages 151-161
    4. Víctor Sánchez-Margalet, José Santos-Alvarez, Sandra Díaz-Troya
      Pages 187-194
    5. Jaime Millán, María C. de Marco, Mohammed Qaidi, Alicia Batista, Fernando Martín-Belmonte, Miguel A. Alonso
      Pages 223-230
    6. Maria João Bonifácio, Patrício Soares-da-Silva
      Pages 231-238
    7. Da-Neng Wang, M. Joanne Lemieux, Jonathan M. Boulter
      Pages 239-255
    8. Jill V. Lyles, Kathleen Cornely-Moss, Christine M. Smith, David L. Daleke
      Pages 257-269
    9. Sathyanarayana N. Gummadi, Sigrún Hrafnsdóttir, Jane Walent, William E. Watkins, Anant K. Menon
      Pages 271-279
  5. Structural Analysis of Membrane Proteins

    1. Front Matter
      Pages 303-303
    2. Valentin I. Gordeliy, Ramona Schlesinger, Rouslan Efremov, Georg Büldt, Joachim Heberle
      Pages 305-316
  6. Back Matter
    Pages 329-334

About this book

Introduction

Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major universities and research centers around the world describe in detail the key techniques that have proven successful in the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
Timely and highly practical, Membrane Protein Protocols: Expression, Purification, and Characterization offers investigators proven methods to determine the three-dimensional structure of membrane receptors and transport proteins, as well as proven practical help in the discovery of new clinical targets for the design of novel pharmaceutical agents useful in treating cancer, bacterial and viral infection, and many metabolic diseases.

Editors and affiliations

  • Barry S. Selinsky
    • 1
  1. 1.Department of ChemistryVillanova UniversityVillanova

Bibliographic information

  • DOI https://doi.org/10.1385/159259400X
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-124-0
  • Online ISBN 978-1-59259-400-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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