Abstract
Concomitant developments in molecular biology and neuroanatomy have permitted the development of techniques to visualize the expression of messenger RNA (mRNA) of interest in its neuroanatomical context and in a relatively quantitative manner. In situ hybridization can be performed using either radioactive or nonradioactive techniques and quantitated using specialized image analysis programs. This chapter will describe a sensitive radioactive method of in situ hybridization, with references given to other variants in technique.
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References
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© 2003 Humana Press Inc.
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Gutstein, H.B. (2003). In Situ Hybridization in Neural Tissues. In: Pan, Z.Z. (eds) Opioid Research. Methods in Molecular Biology™, vol 84. Humana Press. https://doi.org/10.1385/1-59259-379-8:95
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DOI: https://doi.org/10.1385/1-59259-379-8:95
Publisher Name: Humana Press
Print ISBN: 978-1-58829-059-5
Online ISBN: 978-1-59259-379-8
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