Opioid Research

Methods and Protocols

  • Zhizhong Z. Pan

Part of the Methods in Molecular Biology™ book series (MIMM, volume 84)

Table of contents

  1. Front Matter
    Pages i-x
  2. Molecular Characterization of Opioid Receptors and Signaling Pathways

  3. Mapping and Detection of Endogenous Opioids

    1. Front Matter
      Pages 77-77
    2. Howard B. Gutstein
      Pages 95-105
    3. Lionel Moulédous, Howard B. Gutstein
      Pages 141-154
  4. Model Systems for Studies on Opioid Functions

    1. Front Matter
      Pages 155-155
    2. Ivone Gomes, Lakshmi A. Devi, Julija Filipovska
      Pages 157-183
    3. Julija Filipovska, Ivone Gomes, Wei Xu, Chongguang Chen, Lee-Yuan Liu-Chen, Lakshmi A. Devi
      Pages 185-203
    4. Ichiro Sora, Kazutaka Ikeda, Yuji Mishina
      Pages 205-216
    5. Daphné A. Robinson, Min Zhuo
      Pages 217-222
    6. Zhizhong Z. Pan
      Pages 223-232

About this book

Introduction

Opioid research has grown enormously over the last decade, and with it, the need to apply multiple techniques from a variety of disciplines. In Opioid Research: Methods and Protocols, expert researchers and physicians provide a comprehensive collection of the major cross-disciplinary methods and protocols used in current opioid research, covering topics from molecular and genetic techniques, to behavioral analyses of animal models, to clinical practices. The well-practiced authors describe their best molecular techniques for the cloning and expression of opioid receptors, and for the quantitative characterization of their signaling pathways, as well as for mapping the distribution and detecting the expression levels of opioid receptors, opioid peptides, and their messages, in both brain tissues and individual cells. Also included are methods for the creation of in vitro and in vivo animal models to study opioid functions, as well as the clinical applications in the treatment of pain and opioid addiction. Each proven technique contains step-by-step instructions, explanations of the basic science involved, and notes on avoiding pitfalls.
Comprehensive and authoritative, Opioid Research: Methods and Protocols offers researchers an indispensable guide to all the major multidisciplinary techniques needed for successful research in opioid biology and medicine today.

Editors and affiliations

  • Zhizhong Z. Pan
    • 1
  1. 1.Departments of Symptom Research and Biochemistry and Molecular BiologyThe University of Texas MD Anderson Cancer CenterHouston

Bibliographic information

  • DOI https://doi.org/10.1385/1592593798
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-059-5
  • Online ISBN 978-1-59259-379-8
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
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