Abstract
Synthesis of complementary DNA (cDNA) by reverse transcription (RT) is a key step in investigating specific gene expression of a single transcript by RT-PCR (polymerase chain reaction) or to study the more complex profiles of gene expression in a biological sample using cDNA library or other techniques. Solid-phase generation of cDNA (1), based on immobilization of the synthesized cDNA on paramagnetic beads and the magnetic-bead separation technology, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (see Note 1). Using this method, analysis of gene expression can be carried out at a single-cell level (2–4). This is not possible using conventional techniques of mRNA isolation and cDNA cloning.
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© 2003 Humana Press Inc., Totowa, NJ
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Wang, Z., Jones, M.G.K. (2003). cDNA Generation on Paramagnetic Beads. In: Ying, SY. (eds) Generation of cDNA Libraries. Methods in Molecular Biology™, vol 221. Humana Press. https://doi.org/10.1385/1-59259-359-3:25
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DOI: https://doi.org/10.1385/1-59259-359-3:25
Publisher Name: Humana Press
Print ISBN: 978-1-58829-066-3
Online ISBN: 978-1-59259-359-0
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