Skip to main content
SpringerLink
Log in
Book cover

Generation of cDNA Libraries

Methods and Protocols

  • Book
  • © 2003

Overview

Editors:
  1. Shao-Yao Ying

    1. Keck School of Medicine, University of Southern California, Los Angeles

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 221)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Complementary DNA Libraries

    • Shao-Yao Ying
    Pages 1-12

  3. Rapid Amplification of cDNA Ends

    • Yue Zhang
    Pages 13-24

  4. cDNA Generation on Paramagnetic Beads

    • Zhaohui Wang, Michael G. K. Jones
    Pages 25-31

  5. Construction of a Normalized cDNA Library by mRN-cDNA Hybridization and Subtraction

    • Ye-Guang Chen
    Pages 33-40

  6. Amplification of cDNA Ends Using PCR Suppression Effect and Step-Out PCR

    • Mikhail V. Matz, Naila O. Alieva, Alex Chenchik, Sergey Lukyanov
    Pages 41-49

  7. Use of Inverse PCR to Clone cDNA Ends

    • Sheng-He Huang, Steven H. M. Chen, Ambrose Y. Jong
    Pages 51-58

  8. Construction of Size-Fractionated cDNA Library Assisted by an In Vitro Recombination Reaction

    • Osamu Ohara
    Pages 59-71

  9. Construction of a Full-Length Enriched and a 5′-End Enriched cDNA Library Using the Oligo-Capping Method

    • Yutaka Suzuki, Sumio Sugano
    Pages 73-91

  10. cDNA Library Construction Using In Vitro Transcriptional Amplification

    • Shi-Lung Lin, Henry Ji
    Pages 93-101

  11. Amplification of Representative cDNA Pools from Microscopic Amounts of Animal Tissue

    • Mikhail V. Matz
    Pages 103-116

  12. Single-Cell cDNA Library Construction Using Cycling aRNA Amplification

    • Shi-Lung Lin
    Pages 117-127

  13. mRNA/cDNA Library Construction Using RNA—Polymerase Cycling Reaction

    • Shi-Lung Lin, Shao-Yao Ying
    Pages 129-143

  14. Quality Assessment of cDNA Libraries

    • Hans-Jürgen Fülle
    Pages 145-153

  15. Assessment of the Quality of mRNA Libraries by Agarose Gel Electrophoresis

    • Tsen-Yin Lin, Shao-Yao Ying
    Pages 155-159

  16. PACS RT-PCR

    • Igor Nepluev, Rodney J. Folz
    Pages 161-168

  17. Single-Cell mRNA Library Analysis by Northern Blot Hybridization

    • Shi-Lung Lin
    Pages 169-178

  18. Generation of cDNA Libraries for Profiling Gene Expression of Given Tissues or Cells

    • Xin Zhang, Qiu-Hua Huang, Ze-Guang Han
    Pages 179-195

  19. Screening Poly [dA/dT(−)] cDNA for Gene Identification

    • San Ming Wang, Scott C. Fears, Lin Zhang, Jian-Jun Chen, Janet D. Rowley
    Pages 197-205

  20. Generation of Longer cDNA Fragments from SAGE Tags for Gene Identification

    • Jian-Jun Chen, Sanggyu Lee, Guolin Zhou, Janet D. Rowley, San Ming Wang
    Pages 207-222
Back to top

About this book

Since its invention and subsequent development nearly 20 years ago, po- merase chain reaction (PCR) has been extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current decoding and potential application of human genome information to genechips, there are urgent needs for identification of functional significance of these decoded gene sequences. Inherent in bringing these app- cations to fruition is the need to generate a complete and full-length cDNA library for potential functional assays of specific gene sequences. Generation of cDNA Libraries: Methods and Protocols serves as a laboratory manual on the evolution of generation of cDNA libraries, covering both ba- ground information and step-by-step practical laboratory recipes for which p- tocols, reagents, operational tips, instrumentation, and other requirements are detailed. The first chapter of the book is an overview of the basics of generating cDNA libraries, which include the following: (a) the definition of a cDNA library, (b) different kinds of cDNA libraries, (c) differences between methods for cDNA library generation using conventional approaches and novel stra- gies, including reverse generation of RNA repertoires from cDNA libraries, and (d) the quality of cDNA libraries.

Reviews

...the ready-for-use protocols are excellent...A good buy. - Cell Biochem Funct

Editors and Affiliations

  • Keck School of Medicine, University of Southern California, Los Angeles

    Shao-Yao Ying

Bibliographic Information

  • Book Title: Generation of cDNA Libraries

  • Book Subtitle: Methods and Protocols

  • Editors: Shao-Yao Ying

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593593

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-1-58829-066-3Published: 19 February 2003

  • Softcover ISBN: 978-1-61737-333-6Published: 10 November 2010

  • eBook ISBN: 978-1-59259-359-0Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 338

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

Publish with us

Policies and ethics

Back to top

Search

Navigation