Skip to main content
SpringerLink
Log in

Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral Blood

  • Protocol
Cytokines and Colony Stimulating Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 215))

Abstract

Dendritic cells (DCs) are the most potent inducers of immune responses and potent regulators of immunity. They act as sentinel cells in the peripheral tissues, playing a key role in the development of effective immune responses to different types of pathogens (1). At the same time, DC dysfunction may play a pathogenic role in different diseases, ranging from autoimmunity to chronic infections and cancer, and multiple pathogens developed ways to interfere with DC functions as a mean to avoid eradication by the immune system and to enhance their own survival within infected hosts (2).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Banchereau, J. and Steinman, R. M. (1998) Dendritic cells and the control of immunity. Nature 392, 245–252.

    Article  CAS  Google Scholar 

  2. Bhardwaj, N. (1997) Interactions of viruses with dendritic cells: a double-edged sword. J. Exp. Med. 186, 795–799.

    Article  CAS  Google Scholar 

  3. Kalinski, P., Hilkens, C. M., Wierenga, E. A., and Kapsenberg, M. L. (1999) T-cell priming by type-1 and type-2 polarized dendritic cells: the concept of a third signal. Immunol. Today 20, 561–567.

    Article  CAS  Google Scholar 

  4. Moser, M. and Murphy, K. M. (2000) Dendritic cell regulation of TH1-TH2 development. Nat. Immunol. 1, 199–205.

    Article  CAS  Google Scholar 

  5. Pulendran, B., Palucka, K., and Banchereau, J. (2001) Sensing pathogens and tuning immune responses. Science 293, 253–256.

    Article  CAS  Google Scholar 

  6. Liu, Y. J., Kanzler, H., Soumelis, V., and Gilliet, M. (2001) Dendritic cell lineage,plasticity and cross-regulation. Nat. Immunol. 2, 585–589.

    Article  CAS  Google Scholar 

  7. Kalinski, P., Hilkens, C. M., Snijders, A., Snijdewint, F. G., and Kapsenberg, M. L. (1997) IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T-helper cells. J. Immunol. 159, 28–35.

    CAS  Google Scholar 

  8. De Smedt, T., Van Mechelen, M., De Becker, G., Urbain, J., Leo, O., and Moser, M. (1997) Effect of interleukin-10 on dendritic cell maturation and function. Eur.J. Immunol. 27, 1229–1235.

    Article  CAS  Google Scholar 

  9. McRae, B. L., Semnani, R. T., Hayes, M. P., and van Seventer, G. A. (1998) Type I IFNs inhibit human dendritic cell IL-12 production and Th1 cell development. J. Immunol. 160, 4298–4304.

    CAS  Google Scholar 

  10. Kalinski, P., Schuitemaker, J. H., Hilkens, C. M., and Kapsenberg, M. L. (1998) Prostaglandin E2 induces the final maturation of IL-12-deficient CD1a+CD83+dendritic cells: the levels of IL-12 are determined during the final dendritic cell maturation and are resistant to further modulation. J. Immunol. 161, 2804–2809.

    CAS  Google Scholar 

  11. Vieira, P. L., Kalinski, P., Wierenga, E. A., Kapsenberg, M. L., and de Jong, E. C. (1998) Glucocorticoids inhibit bioactive IL-12p70 production by in vitrogenerated human dendritic cells without affecting their T cell stimulatory potential.J. Immunol. 161, 5245–5251.

    CAS  Google Scholar 

  12. de Jong, E. C., Vieira, P. L., Kalinski, P., and Kapsenberg, M. L. (1999) Cortico-steroids inhibit the production of inflammatory mediators in immature monocyte-derived DC and induce the development of tolerogenic DC3. J. Leukoc. Biol. 66, 201–204.

    Article  CAS  Google Scholar 

  13. Vanderheyde, N., Verhasselt, V., Goldman, M., and Willems, F. (1999) Inhibition of human dendritic cell functions by methylprednisolone. Transplantation 67, 1342–1347

    Article  CAS  Google Scholar 

  14. Vieira, P. L., de Jong, E. C., Wierenga, E. A., Kapsenberg, M. L., and Kalinski, P.(2000) Development of Th1-inducing capacity in myeloid dendritic cells requires environmental instruction. J. Immunol. 164, 4507–4512.

    Article  CAS  Google Scholar 

  15. Gagliardi, M. C., Sallusto, F., Marinaro, M., Langenkamp, A., Lanzavecchia, A., and De Magistris, M. T. (2000) Cholera toxin induces maturation of human dendritic cells and licences them for Th2 priming. Eur. J. Immunol. 30, 2394–2403.

    Article  CAS  Google Scholar 

  16. Kalinski, P., Schuitemaker, J. H., Hilkens, C. M., Wierenga, E. A., and Kapsenberg, M. L. (1999) Final maturation of dendritic cells is associated with impaired responsiveness to IFN-gamma and to bacterial IL-12 inducers: decreased ability of mature dendritic cells to produce IL-12 during the interaction with Th cells. J. Immunol. 162, 3231–3236.

    CAS  Google Scholar 

  17. Langenkamp, A., Messi, M., Lanzavecchia, A., and Sallusto, F. (2001) Kinetics of dendritic cell activation: impact on priming of Th1, Th2 and nonpolarized T cells. Nat. Immunol. 1, 311–316.

    Article  Google Scholar 

  18. Kalinski, P, Smits, H. H., Schuitemaker, J. H., Vieira, P. L., van Eijk, M., de Jong, E. C., et al. (2000) IL-4 is a mediator of IL-12p70 induction by human Th2 cells: reversal of polarized Th2 phenotype by dendritic cells. J. Immunol. 165, 1877–1881.

    Article  CAS  Google Scholar 

  19. Peters, J. H., Xu, H., Ruppert, J., Ostermeier, D., Friedrichs, D., and Gieseler, R. K. (1993) Signals required for differentiating dendritic cells from human monocytes in vitro. Adv. Exp. Med. Biol. 329, 275–280.

    Article  CAS  Google Scholar 

  20. Sallusto, F. and Lanzavecchia, A. (1994) Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J. Exp. Med. 179, 1109–1118.

    Article  CAS  Google Scholar 

  21. Jonuleit, H., Kuhn, U., Muller, G., Steinbrink, K., Paragnik, L., Schmitt, E., et al.(1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27, 3135–3142.

    Article  CAS  Google Scholar 

  22. Fraser, J. D. (1989) High-affinity binding of staphylococcal enterotoxins A and B to HLA-DR. Nature 339, 221–223.

    Article  CAS  Google Scholar 

  23. Hilkens, C. M., Kalinski, P., de Boer, M., and Kapsenberg, M. L. (1997) Human dendritic cells require exogenous interleukin-12-inducing factors to direct the development of naive T-helper cells toward the Th1 phenotype. Blood 90, 1920–1926.

    Article  CAS  Google Scholar 

  24. Snijders, A., Kalinski, P., Hilkens, C. M., and Kapsenberg, M. L. (1998) High-level IL-12 production by human dendritic cells requires two signals. Int. Immunol. 10, 1593–1598.

    Article  CAS  Google Scholar 

  25. Kalinski, P., Vieira, P. L., Schuitemaker, J. H., de Jong, E. C., and Kapsenberg, M. L. (2001) Prostaglandin E2 is a selective inducer of IL-12p40 production and an inhibitor of bioactive IL-12p70 heterodimer. Blood 97, 3466–3569.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, University of Pittsburgh, PA, USA

    Pawel Kalinski & Quan Cai

  2. Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, The Netherlands

    Pedro Vieira, Joost H. N. Schuitemaker & Martien Kapsenberg

Authors
  1. Pawel Kalinski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pedro Vieira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joost H. N. Schuitemaker
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Quan Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Martien Kapsenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Pediatrics, University of Leipzig, Leipzig, Germany

    Dieter Körholz  & Wieland Kiess  & 

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Humana Press Inc.

About this protocol

Cite this protocol

Kalinski, P., Vieira, P., Schuitemaker, J.H.N., Cai, Q., Kapsenberg, M. (2003). Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral Blood. In: Körholz, D., Kiess, W. (eds) Cytokines and Colony Stimulating Factors. Methods in Molecular Biology, vol 215. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-345-3:427

Download citation

  • DOI: https://doi.org/10.1385/1-59259-345-3:427

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-58829-035-9

  • Online ISBN: 978-1-59259-345-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation