Abstract
SDS PAGE/immunoblotting is the method of choice for the qualitative and quantitative detection of specific integrins in a given sample. In the simplest case, the cells under investigation are lysed, the lysates separated by SDS PAGE, transferred onto a membrane, and the integrins are then identified by using specific anti-integrin antibodies. If the aim of an experiment is limited to the identification or quantification of a specific integrin subunit in a given sample SDS PAGE/immunoblotting of a cell lysate using specific antibodies yields sufficient information. The advantage of separating and blotting the protein extract onto a membrane compared to a simple detection of a protein of interest, e.g., in an ELISA assay is the additional information about the size of the protein that is detected. This is particularly important when it is not clear if the antibodies used for detection of the protein cross-react with other proteins in the cell lysate.
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© 1999 Humana Press Inc.
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Schneller, M., Arap, W., Pasqualini, R. (1999). Immunoblotting of Integrins. In: Howlett, A. (eds) Integrin Protocols. Methods in Molecular Biology, vol 129. Humana Press. https://doi.org/10.1385/1-59259-249-X:63
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DOI: https://doi.org/10.1385/1-59259-249-X:63
Publisher Name: Humana Press
Print ISBN: 978-0-89603-569-0
Online ISBN: 978-1-59259-249-4
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