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Integrin Protocols

  • Book
  • © 1999

Overview

Editors:
  1. Anthony Howlett

    1. Berlex Biosciences, Richmond, USA

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Part of the book series: Methods in Molecular Biology (MIMB, volume 129)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. Working with Integrins

    1. Front Matter

      Pages 1-1
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    2. Integrin-Binding Peptides Derived from Phage Display Libraries

      • Erkki Koivunen, Bradley H. Restel, Daniel Rajotte, Johanna Lahdenranta, Martin Hagedorn, Wadih Arap et al.
      Pages 3-17

    3. Production of Rat Monoclonal Antibodies Specific for Mouse Integrins

      • Marc Delcommenne, Charles H. Streuli
      Pages 19-34

    4. Biochemical Analysis of Integrin-Mediated Shc Signaling

      • Kishore K. Wary, Michael Dans, Agnese Mariotti, Filippo G. Giancotti
      Pages 35-49

    5. Assays for Kinase Activity

      • Maja H. Oktay, Agnese Mariotti, Filippo G. Giancotti
      Pages 51-61

    6. Immunoblotting of Integrins

      • Maximilian Schneller, Wadih Arap, Renata Pasqualini
      Pages 63-78

    7. Application of Flow Cytometry in the Analysis and Sterile Sorting of Cell Populations Based on Integrin Expression

      • Edward C. Rosfjord, Robert B. Dickson
      Pages 79-90

    8. Expression of Integrin Transcripts in Human Cancer Cells

      • Wadih Arap, H.-J. Su Huang
      Pages 91-101

  3. Functional Analysis of Integrins

    1. Front Matter

      Pages 103-103
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    2. Functional Analysis of the β2 Integrins

      • Rui Li, M. Amin Arnaout
      Pages 105-124

    3. Expression of Heterologous Integrin Genes

      • Craig A. Steger, Mara Fornaro, Amy S. Woodard, Duo-Qi Zheng, Lucia R. Languino
      Pages 125-134

    4. Preparing a Polyclonal Antibody to Mouse β1 Integrin with Function-Blocking Activity

      • Gwynneth M. Edwards, Charles H. Streuli
      Pages 135-152

    5. Integrin Gene Targeting

      • Jan F. Talts, Cord Brakebusch, Reinhard Fässler
      Pages 153-187

    6. Determination of Positive and Negative Regulatory Elements

      • Emilio Hirsch, Piero Cervella, Lorenzo Silengo, Fiorella Altruda
      Pages 189-208

  4. Integrin Function at the Cellular Level

    1. Front Matter

      Pages 209-209
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    2. A Cell-Based Adhesion Assay for the Characterization of Integrin αvβ3 Antagonists

      • James M. Wu, Mary P. Rosser, Anthony R. Howlett, Richard I. Feldman
      Pages 211-217

    3. Integrins in Cell Migration

      • Klaus von der Mark, Stephan Schöber, Simon L. Goodman
      Pages 219-230

    4. Models for Studying Cellular Invasion of Basement Membranes

      • John T. Price, Erik W. Thompson
      Pages 231-249

    5. Methods for Studying Anoikis

      • Steven M. Frisch
      Pages 251-256
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About this book

In Integrin Protocols, Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression-at both the RNA and protein levels-and for elucidating the functional properties of integrins, including those at the cellular level. Each method provides step-by-step instructions for easy reproducibility, along with extensive notes about potential pitfalls, and tips on how to avoid failure. The emphasis is always on the practical steps necessary for experimental success and robust results.
Offering powerful tools for understanding how integrins regulate cell growth, differentiation, migration, invasion, angiogenesis, and apoptosis, as well as how abnormalities of integrin expression and function may be implicated in various pathologic conditions, Integrin Protocols constitutes a gold-standard collection of techniques for both new and experienced investigators of the molecular and cellular basis of cardiovascular disease, inflammatory disorders, and cancer.

Reviews

"Integrin Protocols aims to provide a hand-book of methods for the study of integrins. Many of the essential techniques are covered, with sections ranging from the relatively straight-forward (immunoblotting) to the highly complex (phage display and knock-outs)...Over half of the sections are by major figures in the integrin field, thus giving the book considerable authority..."-Cell Biology International 2000

Editors and Affiliations

  • Berlex Biosciences, Richmond, USA

    Anthony Howlett

Bibliographic Information

  • Book Title: Integrin Protocols

  • Editors: Anthony Howlett

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159259249X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1999

  • Hardcover ISBN: 978-0-89603-569-0Published: 15 July 1999

  • Softcover ISBN: 978-1-4899-4341-5Published: 23 August 2013

  • eBook ISBN: 978-1-59259-249-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 296

  • Number of Illustrations: 24 b/w illustrations

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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