Abstract
Monoclonal antibodies (MAbs) have proved to be an important tool for identifying novel cell-adhesion molecules and for determining their ligand binding specificity, function, and structure. Consequently, they have been instrumental in defining three families of adhesion receptors: the cadherin, immunoglobulin, and integrin families. Integrins include over 20 adhesion receptors that react with the extracellular matrix or cell-surface molecules. Integrins are composed of two distinct transmembrane glycoprotein subunits, α and β, which are noncovalently linked to each other. At least 15 α chains and 8 β chains have been observed in several possible combinations that determine the ligand specificity and function of the complex (1).
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References
Hynes, R. O. (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69, 11–25.
Landis, R. C, Bennett, R. I., and Hogg, N. (1993) A novel LFA-1 activation epitope maps to the I domain. J. Cell Biol. 120, 1519–1527.
Lenter, M., Uhlig, H., Hamann, A., Jeno, P., Imhof, B., and Vestweber, D. (1993) A monoclonal antibody against an activation epitope on mouse integrin chain β1 blocks adhesion of lymphocyes to the endothelial integrin α6β1. Proc. Natl. Acad. Sci. USA 90, 9051–9055.
Mould, A. P., Garratt, A. N., Askari, J. A., Akiyama, S. K., and Humphries, M. J. (1995) Identification of a novel anti-integrin monoclonal antibody that recognises ligand-induced binding site epitope on the α4β1 subunit. FEBS Lett. 363, 118–122.
Woodside, D. G., Teague, T. K., and McIntyre B. W. (1996) Specific inhibition of T lymphocyte coactivation by triggering integrin β1 reveals convergence of β1, β2, and β7 signaling pathways. J. Immunol. 157, 700–706.
Sanchez-Madrid, F. and Springer, T. A. (1986) Production of syrian and armenian hamster monoclonal antibodies of defined specificity. Methods Enzymol. 121, 239–244.
Sonnenberg, A., Daams, H., Van Der Valk, M., Hilkens, J., and Hilgers, J. (1986) Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. J. Histochem Histochem. 34, 1037–1046.
Delcommenne, M. and Streuli, C. H. (1995) Control of integrin expression by the extracellular matrix. J. Biol. Chem. 270, 26,794–26,801.
Milner, R., Frost, E., Nishimura, S., Delcommenne, M., Streuli, C. H., Pytela, R., and ffrench-Constant, C. (1997) Expression of αvβ3 and αvβ8 integrins during oligodendrocyte precursor differentiation in the presence and absence of axons. Glia 21, 350–360.
De Clercq, L., Cormont, F., and Bazin, H. (1986) Generation of rat-rat hybridomas with the use of the LOU IR983F non secreting fusion cell line. Methods Enzymol. 121, 234–244.
Long, W. J., McGuire, W., Palombo, A., and Emini, E. A. (1986) Enhancing the establishment and efficiency of hybridoma cells. Use of irradiated human diploid fibroblast feeder layers. J. Immunol. Methods 86, 89–93.
Galfrè, G and Milstein, C. (1981) Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 73B, 3–46.
Pont, S., Naquet, P., Marchetto, S., Regnier-Vigouroux, A., Blanc, D., Pierres, A., and Pierres, M. (1986). Identification of 5 topographic domains of the mouse LFA-1 molecule: subunit assignment and functional involvement in lymphoid cell interactions. J. Immunol. 136, 3750–3759.
Maxfield, S. R., Moulder, K. Koning, F., Elbe, A., Stingl, G., Coligan J. E., Shevach, E. M., and Yokoyama, W. M. (1989). Murine T cells express a cell surface receptor for multiple extracellular matrix proteins. Identification and characterization with monoclonal antibodies. J. Exp. Med. 169, 2173–2190.
Kilshaw, P. J. and Murant, S. J. (1991) Expression and regulation of β7 (βp) integrins on mouse lymphocytes: relevance to the mucosal immune system. Eur. J. Immunol. 21, 2591–2597.
Santini, G. F., Battistin, S., De Paoli, P., Villalta, D., Borean, M., and Basaglia, G (1987) A method for the determination of the adherence of granulocytes to microtitre plates. J. Immunol. Meth. 100, 153–156.
Miyake, S., Sakkurai, T., Odumura, K., and Yagita, H. (1988). Identification of collagen and laminin receptor integrins on murine T lymphocytes. Eur. J. Immunol. 249, 2000–2005.
Hurley, W. L., Finkelstein, E., and Holst, B. D. (1985) Identification of surface proteins on bovine leukocytes by a biotin-avidin protein blotting techniques. J. Immunol. Nethods 85, 195–202
Neff, N. T., Lowrey, C., Decker, C., Tovar, A., Damsky, C., Buck, C., and Horwitz, A. F. (1982) A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices. J. Cell Biol. 95, 654–666.
Davignon, D., Marz, E., Reynolds, T., Kürzinger, K., and Springer, T. A. (1981) Monoclonal antibody to a novel lymphocyte function-associated antigen (LFA-1): mechanism of blockade of T lymphocyte-mediated killing and effects on other T and B lymphocyte functions. J. Immunol. 127, 590–595.
Arnaout, M. A., Todd, R. F., III, Dana, N., Melamed, J., Schlossman, S. F., and Colten, H. R. (1983) Inhibition of phagocytosis of complement C3-or immmunoglobulin G-coated particles and of C3bi binding by monoclonal antibodies to a monocyte-granulocyte membrane glycoprotein (Mo1). J. Clin. Invest. 72, 171–179.
Coller, B. S., Peerschke, E. I., Scudder, L. E., and Sullivan, C. A. (1982) A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycopro-teins IIb and/or IIIa. J. Clin. Invest. 72, 325–338.
Springer, T., Galfré, G., Secher, D. S., and Milstein, C. (1979) Mac-1: a macro-phage differentiation antigen identified by monoclonal antibody. Eur. J. Immunol. 9, 301–306.
Miyake, K., Weissman, H., Greenberger, J. S., and Kincade, P. W. (1988). Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. (1991) J. Exp. Med. 173, 599–607.
Lokeshwar, B. L. and Lin, H. S. (1988) Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor. J. Immunol. 141, 483–488.
Taggart, R. T., Samloff, I. M. (1982) Stable antibody-producing murine hybridomas. Science 219, 1228–1230.
Walker, K. Z., Gibson, J., Axiak, S. M., and Prentice, R. L. (1986) Potentiation of hybridoma production by the use of mouse fibroblast conditioned media. J. Immunol. Meth. 88, 75–81.
Gomathi, K. G., Sharma, E., and Sharma, S. K. (1991) Protein growth factor(s) from C6 glioma cells that promote the growth of murine hybridomas. J. Immunol. Meth. 139, 101–105.
Yamamoto, R., Lin, L. S., Warren, M. K., and White, T. J. (1990) The human lung fibroblast cell line, MRC-5, produces multiple factors involved with megakaryocytopoiesis. J. Immunol. 144, 1808–1816.
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© 1999 Humana Press Inc.
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Delcommenne, M., Streuli, C.H. (1999). Production of Rat Monoclonal Antibodies Specific for Mouse Integrins. In: Howlett, A. (eds) Integrin Protocols. Methods in Molecular Biology, vol 129. Humana Press. https://doi.org/10.1385/1-59259-249-X:19
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DOI: https://doi.org/10.1385/1-59259-249-X:19
Publisher Name: Humana Press
Print ISBN: 978-0-89603-569-0
Online ISBN: 978-1-59259-249-4
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