Abstract
There are a number of proteins involved in DNA replication, recombination, or repair that bind stoichiometrically to single DNA strands irrespective of the nucleotide sequence, and some of these proteins also bind to single-stranded RNA. Some of the best known examples are the ssb protein of Escherichia coli, the gene 32 protein of phage T4, the gene 5 protein of the M13/fd/f1 filamentous bacterial viruses, and the more recently isolated human replication protein A (1–4). Complexes formed by these proteins contain protein bound to the nucleic acid at defined ratios of the number of nucleotides per molecule of bound protein; the ratios are determined by the interactive properties of the protein. These ratios, and the structures of the complexes that are formed, may vary with factors such as changes in solution conditions that alter the binding properties of the proteins.
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© 2001 Humana Press Inc., Totowa, NJ
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Gray, C.W. (2001). Electron Microscopy of Protein-Nucleic Acid Complexes. In: Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology, vol 148. Humana Press. https://doi.org/10.1385/1-59259-208-2:579
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DOI: https://doi.org/10.1385/1-59259-208-2:579
Publisher Name: Humana Press
Print ISBN: 978-0-89603-625-3
Online ISBN: 978-1-59259-208-1
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