Abstract
Technological advancement in molecular biology set in motion the directed evolution of the properties of biomolecules, such as protein and nucleic acids. Toward the improvement of the properties in general, events in nature are imitated. The approach, commonly called “directed evolution,” involves the alternating process of diversification, which is the generation of libraries, and subsequent selection for the fittest. The serial cycles of consecutive diversification and selection processes eventually lead to the accumulation of beneficial mutations, driving the improvement of targeted properties.
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© 2002 Humana Press Inc.
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Matsuura, T., Yomo, T., Urabe, I. (2002). Evolutionary Molecular Engineering by Random Elongation Mutagenesis. In: Braman, J. (eds) In Vitro Mutagenesis Protocols. Methods in Molecular Biology™, vol 182. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-194-9:221
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DOI: https://doi.org/10.1385/1-59259-194-9:221
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-910-0
Online ISBN: 978-1-59259-194-7
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