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In Vitro Mutagenesis Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Jeff Braman

    1. Stratagene, La Jolla

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Part of the book series: Methods in Molecular Biology (MIMB, volume 182)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Rapid and Reliable Site-Directed Mutagenesis Using Kunkel’s Approach

    • Priya Handa, Swapna Thanedar, Umesh Varshney
    Pages 1-6

  3. Site-Directed Mutagenesis Using Altered /gb-Lactamase Specificity

    • Christine A. Andrews, Scott A. Lesley
    Pages 7-17

  4. Site-Directed Mutagenesis Facilitated by DpnI Selection on Hemimethylated DNA

    • Fusheng Li, James I. Mullins
    Pages 19-27

  5. Multiple Site-Directed Mutagenesis In Vitro

    • Yang-Gyun Kim, Stefan Maas
    Pages 29-36

  6. Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikChangeTM Site-Directed Mutagenesis

    • Wenyan Wang, Bruce A. Malcolm
    Pages 37-43

  7. Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids

    • Susan A. Nadin-Davis
    Pages 45-54

  8. Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis

    • Grace DeSantis, J. Bryan Jones
    Pages 55-65

  9. Site-Directed Mutagenesis Mediated by a Single Polymerase Chain Reaction Product

    • Xueni Chen, Weimin Liu, Ileana Quinto, Giuseppe Scala
    Pages 67-70

  10. Megaprimer Method for Polymerase Chain Reaction-Mediated Generation of Specific Mutations in DNA

    • Jesper Brøs-Poulsen, Jane Nøhr, Leif Kongskov Larsen
    Pages 71-76

  11. Generation of Epitope-Tagged Proteins by Inverse Polymerase Chain Reaction Mutagenesis

    • Lucio Gama, Gerda E. Breitwieser
    Pages 77-83

  12. Site-Directed Mutagenesis by Polymerase Chain Reaction

    • Albert Jeltsch, Thomas Lanio
    Pages 85-94

  13. Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction

    • Amom Ruhikanta Meetei, M. R. S. Rao
    Pages 95-102

  14. Phenotypic Expression of Polymerase Chain Reaction-Generated Random Mutations in a Foreign Gene After its Introduction into an Acinetobacter Chromosome by Natural Transformation

    • David M. Young, Ruben G. Kok, L. Nicholas Ornston
    Pages 103-115

  15. Polymerase Chain Reaction-Mediated Mutagenesis in Sequences Resistant to Homogeneous Amplification

    • Ross N. Nazar, P. D. Abeyrathne, Robert V. A. Intine
    Pages 117-126

  16. Polymerase Chain Reaction-Based Signature-Tagged Mutagenesis

    • Dario E. Lehoux, Roger C. Levesque
    Pages 127-137

  17. High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction

    • Stefan Howorka, Hagan Bayley
    Pages 139-147

  18. In Vitro Scanning-Saturation Mutagenesis

    • Jennifer A. Maynard, Gang Chen, George Georgiou, Brent L. Iverson
    Pages 149-163

  19. Random Transposon Mutagenesis of Large DNA Molecules in Escherichia coli

    • Wolfram Brune
    Pages 165-171

  20. Random Chromosomal Gene Disruption Using Cassette Mutagenesis

    • French A. Lewis, Brian A. Dougherty
    Pages 173-187
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About this book

In the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. Each contributor focuses on improvements to conventional site-directed mutagenesis, with chapters being devoted to chemical site-directed mutagenesis; PCR-based mutagenesis and the modifications that allow high-throughput experiments; and mutagenesis based on gene disruption that is both in vitro- and in situ-based. Additional methods are provided for in vitro gene evolution; for gene disruption based on transposon, recombination, and cassette mutagenesis; and for facilitating the introduction of multiple mutations. Each readily reproducible technique includes detailed step-by-step instructions, tips on pitfalls to avoid, and notes on reagents and suppliers.


Time-tested and highly practical, the techniques in In Vitro Mutagenesis Protocols, Second Edition offer today's molecular biologists a rich compendium of reliable and powerful techniques with which to illuminate the proteome.

Reviews

"...strongly recommended to scientists who anticipate sophisticated exploitation of mutagenesis techniques in genetic engineering." - Folia Microbiologica

Editors and Affiliations

  • Stratagene, La Jolla

    Jeff Braman

Bibliographic Information

  • Book Title: In Vitro Mutagenesis Protocols

  • Editors: Jeff Braman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591949

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • eBook ISBN: 978-1-59259-194-7Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 288

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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