Abstract
Site-directed in vitro mutagenesis is a powerful tool for investigating the structure-function relationships of proteins and the expression of genes. Often, it is desirable to subject the protein or gene of interest to scanning mutagenesis. The genetic analysis of scores of single or multiple point mutations contributes to a better understanding of cis elements in gene expression (1). In the case of proteins, cysteine (Cys) (2,3) and alanine scanning mutagenesis (4) are popular means for investigating the structure and function of proteins and the interactions between proteins. Cys-scanning mutagenesis is particularly useful, since the introduction of Cys allows targeted chemical modification with a wide variety of sulfhydryl-reactive reagents (5,6).
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Howorka, S., Bayley, H. (2002). High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction. In: Braman, J. (eds) In Vitro Mutagenesis Protocols. Methods in Molecular Biology™, vol 182. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-194-9:139
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DOI: https://doi.org/10.1385/1-59259-194-9:139
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-910-0
Online ISBN: 978-1-59259-194-7
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