Abstract
Neutrophils are manufactured in the bone marrow from stem cell precursors (myeloblasts) by a series of cell divisions. In healthy individuals only the mature neutrophils enter peripheral blood. They are the most numerous leukocyte subgroup, constituting half of the circulating white cell population. Healthy blood donors typically yield 1 million neutrophils per mL of venous blood.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Baggiolini, M. (1980). The Cell Biology of Inflammation (Weissman, G., ed.), Elsevier/North Holland, NY, pp. 163–187.
Aswanikumar, S., Corcoran, B., Schiffman, E., Day, A. R., Freer, R. J., Showell, H. J., and Becker, E. L. (1977) Demonstration of a receptor on rabbit neutrophils for chemotactic peptides. Biochem. Biophys. Res. Comm. 74, 810–817.
Grob, P. M., David, E., Warren, T. C., DeLeon, R. P., Farina, P. R., and Homon, C.A. (1990) Characterisation of a receptor for human monocyte-derived neutrophil chemotactic factor/ interleukin-8. J. Biol. Chem. 265, 8311–8316.
Gerard, N.P. and Gerard, C. (1991) The chemotactic receptor for human C5a anaphylatoxin. Nature. 349, 614–617.
Kreisle, R. A. and Parker, C. W. (1983) Specific binding of leukotriene B4 to a receptor on human polymorphonuclear leukocytes. J. Exp. Med. 157, 628–641.
Kunz, D., Gerard, N. P., and Gerard, C. (1992) The human leukocyte plateletactivating factor receptor. J. Biol. Chem. 267, 9101–9106.
Falk, W., GoodwinJR., R. H., and Leonard, E. J. (1980) A 48-well micro-chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J. Immunol. Methods 33, 239–247.
Wilkinson, P. C. (1988) Micropore filter methods for leukocyte chemotaxis. Methods Enzymol. 162, 38–50.
Allport, J. R., Donnelly, L. E., Hayes, B. P., Murray, S., Rendell, N. B., Ray, K. P., and MacDermot, J. M. (1996) Reduction by inhibitors of mo≩ (ADP-ribosyl) transferase of chemotaxis in human neutrophil leucocytes by inhibition of the assembly of filamentous actin. Br. J. Pharmacol. 118, 1111–1118.
Wolf, H., Fruhwirth, M., Ruedl, C., Oswald, H. P., Fischer, H., Bock, G., and Wick, G. (1995) Chronic granulomatous disease assessed by single call granulocyte oxidative burst activity. Int. Arch. Allergy Immunol. 106(4) 425–427.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Culpitt, S.V. (2001). Neutrophils. In: Rogers, D.F., Donnelly, L.E. (eds) Human Airway Inflammation. Methods in Molecular Medicine, vol 56. Humana Press. https://doi.org/10.1385/1-59259-151-5:177
Download citation
DOI: https://doi.org/10.1385/1-59259-151-5:177
Publisher Name: Humana Press
Print ISBN: 978-0-89603-923-0
Online ISBN: 978-1-59259-151-0
eBook Packages: Springer Protocols