Human Airway Inflammation

Sampling Techniques and Analytical Protocols

  • Duncan F. Rogers
  • Louise E. Donnelly

Part of the Methods in Molecular Medicine book series (MIMM, volume 56)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Airway Inflammation and Remodeling in Asthma

    1. Stephen T. Holgate
      Pages 1-16
  3. Sample Collection

    1. Front Matter
      Pages 17-17
    2. Marina Saetta, Graziella Turato
      Pages 19-29
    3. Chris Ward, E. Haydn Walters
      Pages 31-59
    4. Lennart Greiff, Morgan Andersson, Carl G.A. Persson
      Pages 61-73
  4. Sample Collection

    1. Front Matter
      Pages 75-75
    2. Margaret M. Kelly, Ann Efthimiadis, Frederick E. Hargreave
      Pages 77-91
    3. Vera M. Keatings, Julia A. Nightingale
      Pages 93-98
    4. Paolo Paredi, Sergei A. Kharitonov, Peter J. Barnes
      Pages 109-118
    5. Johan C. de Jongste, Rijn Jöbsis, H. Rolien Raatgeep
      Pages 119-124
  5. Cell Isolation and Culture

    1. Front Matter
      Pages 125-125
    2. Louise E. Donnelly
      Pages 127-136
    3. Ian R. Witherden, Teresa D. Tetley
      Pages 137-146
    4. Joachim Seybold, Norbert Suttorp
      Pages 147-154
    5. Reynold A. Panettieri Jr.
      Pages 155-160
    6. Bernhard F. Gibbs, Madeleine Ennis
      Pages 161-176
    7. Sarah V. Culpitt
      Pages 177-189
    8. Leonard W. Poulter, C. M. Burke
      Pages 191-203

About this book


Airway inflammation is now recognized as central to the pathophysiology of several major respiratory conditions-the two most common being asthma and chronic obstructive pulmonary disease (COPD). In Human Airway Inflammation: Sampling Techniques and Analytical Protocols, experienced basic and clinical investigators describe a wide range of readily reproducible methods for sampling, isolating, and culturing all of the major inflammatory cells involved in the pathophysiology of asthma and COPD. The collection techniques detailed here involve biopsy and sampling of airway liquids such as sputum and exhaled gases such as nitric oxide. Also provided is a full range of methods for the isolation and characterization of cells and for the measurement of many of the major inflammatory markers, along with protocols for measuring the inflammatory mediators and enzymes released during lung inflammation. Each user-friendly method contains all the essential step-by-step elements the researcher must navigate from sample collection to analysis, and includes notes on how to avoid problems. Among the more advanced techniques are the measurement of exhaled hydrocarbons and F2 isoprostanes, granulocyte pharmacodynamics in whole blood measured by flow cytometry, and tracing mediator trafficking in eosinophils using confocal microscopy.
Comprehensive and highly practical, the methods presented in Human Airway Inflammation: Sampling Techniques and Analytical Protocols provide today's basic and clinical researchers with all the major techniques for investigating airway inflammation, and powerfully illuminate many novel targets for emerging drugs.

Editors and affiliations

  • Duncan F. Rogers
    • 1
  • Louise E. Donnelly
    • 1
  1. 1.National Heart and Lung InstituteImperial CollegeLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-923-0
  • Online ISBN 978-1-59259-151-0
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
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