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Development of Replication-Defective Herpes Simplex Virus Vectors

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Gene Therapy Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 69))

Abstract

A greater understanding of the molecular, biochemical, and genetic factors involved in the progression of a specific disease state has led to the development of genetic therapies using direct gene transfer to ameliorate the disease condition or correct a genetic defect in situ. Effective gene therapy approaches require delivery strategies and vehicles that 1) efficiently deliver the therapeutic gene(s) to a sufficient number of dividing or nondividing cells to achieve the desired therapeutic effect; 2) persist long term within the cell without disturbing host cell functions; and 3) can regulate the level and duration of therapeutic gene expression for diseases that may either require high-level transient transgene expression or continuous low-level synthesis of the therapeutic product. Numerous viral and nonviral vectors have been employed to treat a variety of genetic and acquired diseases. Each vector system has its own particular advantages and disadvantages that will suit it to a specific therapeutic application.

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Author information

Authors and Affiliations

  1. Department of Molecular Genetics and Biochemistry, Pittsbugh, PA

    William F. Goins, David M. Krisky, Darren P. Wolfe, David J. Fink & Joseph C. Glorioso

Authors
  1. William F. Goins
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  2. David M. Krisky
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  3. Darren P. Wolfe
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  4. David J. Fink
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  5. Joseph C. Glorioso
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Editor information

Editors and Affiliations

  1. Brown University, Providence, RI

    Jeffrey R. Morgan

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© 2002 Humana Press Inc.

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Goins, W.F., Krisky, D.M., Wolfe, D.P., Fink, D.J., Glorioso, J.C. (2002). Development of Replication-Defective Herpes Simplex Virus Vectors. In: Morgan, J.R. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 69. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-141-8:481

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  • DOI: https://doi.org/10.1385/1-59259-141-8:481

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-723-6

  • Online ISBN: 978-1-59259-141-1

  • eBook Packages: Springer Protocols

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