Abstract
Adenoviruses (Ads) possess several features that make them attractive as mammalian gene transfer vectors. They can efficiently infect a wide variety of quiescent and proliferating cell types from various species to direct high-level viral gene expression, their 36-kb double-stranded DNA genome can be manipulated with relative ease by conventional molecular biology techniques, and they can be readily propagated and purified to yield high-titer preparations of very stable virus. Consequently, Ads have been extensively used as vectors for recombinant vaccines, for high-level protein production in cultured cells and for gene therapy (1–4).
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Ng, P., Graham, F.L. (2002). Construction of First-Generation Adenoviral Vectors. In: Morgan, J.R. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 69. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-141-8:389
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DOI: https://doi.org/10.1385/1-59259-141-8:389
Publisher Name: Springer, Totowa, NJ
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