Abstract
Vector systems based on human immunodeficiency viruses (HIVs) (1) allow an efficient and stable gene transfer into nondividing cells (2,3). Because of the pathogenicity of the parental virus, the use of lentiviral vectors based on feline immunodeficiency virus (4,5), equine infectious anemia virus (6), or simian immunodeficiency virus (SIV) (7,8) has also been explored. SIV is closely related to HIV, and some strains of SIV can induce an AIDS-like disease in nonhuman primates. This animal model has been extensively used to study the pathogenesis of immunodeficiency virus infections and should also be valuable for a preclinical safety assessment of SIV-based vectors. Although SIV can replicate in human CD4+ cells, the course of laboratory-acquired infections suggests greatly reduced virulence in comparison with HIV (9).
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References
Poznansky M., Lever A., Bergeron L., Haseltine W., and Sodroski J. (1991) Gene transfer into human lymphocytes by a defective human immunodeficiency virus type 1 vector. J. Virol. 65, 532–536.
Naldini L., Blömer U., Gallay P., et al. (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263–267.
Reiser J., Harmison G., Kluepfel Stahl S., et al. (1996) Transduction of nondividing cells using pseudotyped defective high-titer HIV type 1 particles. Proc. Natl. Acad. Set USA 93, 15266–15271.
Poeschla E. M., Wong-Staal F., and Looney D. J. (1998) Efficient transduction of nondividing human cells by feline immunodeficiency virus lentiviral vectors. Nat. Med. 4, 354–357.
Johnston J. C., Gasmi M., Lim L. E., et al. (1999) Minimum requirements for efficient transduction of dividing and nondividing cells by feline immunodeficiency virus vectors. J. Virol. 73, 4991–5000.
Mitrophanous K., Yoon S., Rohll J., et al. (1999) Stable gene transfer to the nervous system using a non-primate lentiviral vector. Gene Ther. 6, 1808–1818.
Schnell T., Foley P., Wirth M., Münch J., and Überla K. (2000) Development of a self-inactivating, minimal lentivirus vector based on simian immunodeficiency virus. Hum. Gene Ther. 11, 439–447.
Mangeot P. E., Negre D., Dubois B., et al. (2000) Development of minimal lentivirus vectors derived from simian immunodeficiency virus (SIVmac251) and their use for gene transfer into human dendritic cells. J. Virol. 74, 8307–8315.
Khabbaz R. F., Heneine W., George J.R., et al. (1994) Brief report: infection of a laboratory worker with simian immunodeficiency virus. N. Engl. J. Med. 330, 172–177.
Tang H., Kuhen K. L., and Wong-Staal F. (1999) Lentivirus replication and regulation. Annu. Rev. Genet. 33, 133–370.
Emerman M. and Malim M. H. (1998) HIV-1 regulatory/accessory genes: keys to unraveling viral and host cell biology. Science 280, 1880–1884.
Yee J. K., Miyanohara A., LaPorte P., et al. (1994) A general method for the generation of high-titer, pantropic retroviral vectors: highly efficient infection of primary hepatocytes. Proc. Natl. Acad. Sci. USA 91, 9564–9568.
Wagner R., Graf M., Bieler K., et al. (2000) Rev-independent expression of synthetic gag-pol genes of HIV-1 and SIV: implications for the safety of lentiviral vectors. Hum. Gene Ther. 11, 2403–2413.
Haas J., Park E. C., and Seed B. (1996) Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6, 315–324.
Schneider R., Campbell M., Nasioulas G., Felber B. K., and Pavlakis G. N. (1997) Inactivation of the human immunodeficiency virus type 1 inhibitory elements allows Rev-independent expression of Gag and Gag/protease and particle formation. J. Virol. 71, 4892–4903.
Cullen B. R. (1986) Trans-activation of human immunodeficiency virus occurs via a bimodal mechanism. Cell 46, 973–982.
Malim M. H., Hauber J., Fenrick R., and Cullen B. R. (1988) Immunodeficiency virus rev trans-activator modulates the expression of the viral regulatory genes. Nature 335, 181–183.
DuBridge R. B., Tang P., Hsia H. C., et al. (1987) Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol. Cell Biol. 7, 379–387.
Yee J. K., Friedmann T., and Burns J. C. (1994). Generation of high-titer pseudotyped retroviral vectors with very broad host range, in Methods in Cell Biology, vol. 43, Academic New York, pp. 99–112.
Fouchier R. A. M., Meyer B. E., Simon J. H. M., Fischer U., and Malim M. H. (1997) HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import. EMBO J. 16, 4531–4539.
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Überla, K. (2002). Lentivirus Vector Based on Simian Immunodeficiency Virus. In: Morgan, J.R. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 69. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-141-8:351
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DOI: https://doi.org/10.1385/1-59259-141-8:351
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-723-6
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