Abstract
Microsatellites are tandem repeats of simple sequence, 2–6 bp, that occur abundantly and at random throughout most eukaryotic genomes. They are typically short, often <100 bp long, and are embedded within a unique sequence. Thus, they are ideal for in vitro amplification by the polymerase chain reaction (PCR). The high degree of polymorphism, owing to variation in the number of repeat units, and the stability displayed by micros atellites make them perfect markers for use in constructing high-resolution genetic maps to identify susceptibility loci involved in common genetic diseases (1). In addition to their applications in genome mapping and positional cloning, these markers have been applied in fields as diverse as tumor biology, forensic identification, population genetic analysis, and the construction of human evolutionary trees. This chapter is confined to the analysis of chromosomal deletions.
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References
Koreth, J., O’Leary, J. J., and McGee J. (1996) Microsatellites and PCR genomic analysis. J. Pathol. 178, 239–248.
Dalla-Favera, R., Bregni, M., Erikson, J., Patterson, D., Gallo, R. C., and Croce, C. M. (1982) Human c-myc oncogene is located in the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79, 7824–7827.
Lee, W.-H., Bookstein, R., Hong, F., Young, L.-J., Shew, J.-Y., and Lee, E. Y. (1987) Human retinoblastoma susceptibility gene: cloning, identification and sequencing. Science 235, 1394–1399.
Quelle, D. E., Zindy, F., Ashmun, R. A., and Sherr, C. J. (1995) Alternative reading frames of the INK4a tumour suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest. Cell 83, 993–1000.
Corcoran, M. M., Rasool, O., Liu, Y., Iyengar, A., Grander, D., Ibbotson, R. E., et al. (1998) Detailed molecular delineation of 13q14.3 loss in B-cell chronic lympho cytic leukaemia. Blood 91, 1382–1390.
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© 2002 Humana Press Inc.
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Ibbotson, R.E., Corcoran, M.M. (2002). Detection of Chromosomal Deletions by Microsatellite Analysis. In: Boultwood, J., Fidler, C. (eds) Molecular Analysis of Cancer. Methods in Molecular Medicine, vol 68. Humana Press. https://doi.org/10.1385/1-59259-135-3:059
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DOI: https://doi.org/10.1385/1-59259-135-3:059
Publisher Name: Humana Press
Print ISBN: 978-0-89603-622-2
Online ISBN: 978-1-59259-135-0
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