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Cotransfection Assays and Steroid Receptor Biology

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Steroid Receptor Methods

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 176))

Abstract

The glucocorticoid, mineralocorticoid, progesterone, androgen, estrogen α and estrogen β receptors (GR, MR, PR, AR, ERα, and ERβ, respectively) form the steroid receptor family, part of the nuclear receptor superfamily (1). Like other nuclear receptors, steroid receptors have a conserved domain structure that consists of a C-terminal hormone-binding domain, a central DNA-binding domain, and an N-terminal transcriptional modulatory domain (2). However, unlike other nuclear receptors, in the absence of hormone they are associated with chaperone proteins such as HSP90 (3). Upon binding of steroid, these receptors undergo a conformational change that brings about dissociation of the receptor-chaperone complex, which in turn allows the receptor to bind to DNA, interact with transcriptional coactivators, and activate transcription (4).

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Author information

Authors and Affiliations

  1. American Registry of Pathology at Armed Forces Institute of Pathology, Washington, DC

    Shimin Zhang

  2. Department of Biochemistry and Molecular Biology, Georgetown University, Washington, DC

    Mark Danielsen

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  1. Shimin Zhang
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  2. Mark Danielsen
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Editors and Affiliations

  1. Blueprint Technologies, Englewood, CO

    Benjamin A. Lieberman

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© 2001 Humana Press Inc.

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Zhang, S., Danielsen, M. (2001). Cotransfection Assays and Steroid Receptor Biology. In: Lieberman, B.A. (eds) Steroid Receptor Methods. Methods in Molecular Biology™, vol 176. Humana Press. https://doi.org/10.1385/1-59259-115-9:297

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  • DOI: https://doi.org/10.1385/1-59259-115-9:297

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-754-0

  • Online ISBN: 978-1-59259-115-2

  • eBook Packages: Springer Protocols

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