Steroid Receptor Methods

Protocols and Assays

  • Benjamin A. Lieberman

Part of the Methods in Molecular Biology™ book series (MIMB, volume 176)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Bioinformatics

    1. Front Matter
      Pages 1-36
    2. Mark Danielsen
      Pages 3-22
    3. Llewellyn D. Densmore III
      Pages 23-36
  3. Purification Protocols

    1. Front Matter
      Pages 37-129
    2. Terace M. Fletcher, Barbour S. Warren, Christopher T. Baumann, Gordon L. Hager
      Pages 55-65
    3. Li-Zhi Mi, Fraydoon Rastinejad
      Pages 81-90
    4. Scott A. Chasse, Fraydoon Rastinejad
      Pages 91-103
    5. Sushma Kumar, Maarit Angervo, Milan K. Bagchi, Indrani C. Bagchi
      Pages 105-117
  4. Steroid Hormone-Binding Assays

    1. Front Matter
      Pages 131-175
    2. Rosemary Bland, Martin Hewison
      Pages 145-162
    3. Sami Väisänen, Sanna Ryhänen, Pekka H. Mäenpää
      Pages 163-175
  5. Protein Interaction Assays

    1. Front Matter
      Pages 177-325
    2. Per Flodby, Stephan Teglund, Jan-Åke Gustafsson
      Pages 201-226
    3. Bertrand Le Douarin, David M. Heery, Claudine Gaudon, Elmar vom Baur, Régine Losson
      Pages 227-248

About this book

Introduction

Research into steroid receptor biology holds great promise for the treatment of cancer and endocrine disorders and for elucidating the fundamental biological mechanisms of gene expression. In Steroid Receptor Methods: Protocols and Assays, a distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA-binding characteristics and hormone-binding assays and with the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness. Finally, a discussion by example presents the most current bioinformatics tools and analytical techniques.
Timely and highly accessible, Steroid Receptor Methods: Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery.

Editors and affiliations

  • Benjamin A. Lieberman
    • 1
  1. 1.Blueprint TechnologiesEnglewood

Bibliographic information

  • DOI https://doi.org/10.1385/1592591159
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-754-0
  • Online ISBN 978-1-59259-115-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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