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Extraction and Quantification of the Neurotrophins

  • Protocol
Neurotrophin Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 169))

Abstract

Neurotrophins are a family of neuronal survival and differentiation factors (1,2). The mammalian neurotrophin family consists of four members sharing 50–55% amino acid identity: nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), and NT-4/5 (2,3). Significant interest surrounds the possible use of neurotrophins for the potential treatment of patients with various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer’s disease, and Parkinson’s disease, and for lesioned neurons after traumatic injury (bi4). However, successful therapy requires more knowledge of the physiology of the neurotrophins, and precise, reliable determinations of endogenous neurotrophin protein levels are essential for progress in this area.

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Author information

Authors and Affiliations

  1. Department of Human Physiology and Centre of Neuroscience, Flinders University, Adelaide, Australia

    Shu-Hua Zhang, Xin-Fu Zhou & Robert A. Rush

Authors
  1. Shu-Hua Zhang
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  2. Xin-Fu Zhou
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  3. Robert A. Rush
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Editor information

Editors and Affiliations

  1. Department of Human Physiology, Flinders University, Adelaide, Australia

    Robert A. Rush

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© 2001 Humana Press Inc.

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Zhang, SH., Zhou, XF., Rush, R.A. (2001). Extraction and Quantification of the Neurotrophins. In: Rush, R.A. (eds) Neurotrophin Protocols. Methods in Molecular Biology™, vol 169. Humana Press. https://doi.org/10.1385/1-59259-060-8:31

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  • DOI: https://doi.org/10.1385/1-59259-060-8:31

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-699-4

  • Online ISBN: 978-1-59259-060-5

  • eBook Packages: Springer Protocols

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